沉默MYB基因调控NF-κB信号通路对舌鳞癌细胞生长的抑制作用
| 作者: |
1许炜,
1陶华,
1张琪
1 仙桃市第一人民医院口腔科，湖北 仙桃 433000 |
| 通讯: |
许炜
Email: xuwei0338@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.11.008 |
摘要
目的：探究成髓细胞瘤癌基因(myeloblastoma oncogene，MYB)在舌鳞癌发生、发展中的作用以及相关作用机制。方法：通过荧光定量PCR(real-time PCR，qPCR)检测MYB在舌鳞癌细胞系(UM1，CAL-27，SCC-9)以及正常人口腔黏膜成纤维细胞(oral mucosal fibroblasts，OMFbs)中的表达情况。转染siRNA-MYB至SCC-9细胞中，采用四甲基偶氮唑(MTT)和Transwell法检测其对细胞活性、侵袭、迁移的影响。Western印迹观察核因子-κB(nuclear factor-κB，NF-κB)信号通路的变化。结果：MYB在舌鳞癌细胞系(UM1，CAL-27，SCC-9)细胞中的表达量明显高于正常人OMFbs(P<0.05)。沉默MYB可显著抑制SCC-9细胞的活性、侵袭、迁移(P<0.05)。Western印迹显示沉默MYB表达可显著抑制NF-κB磷酸化，降低基质金属蛋白酶-9(matrix metalloproteinase-9，MMP-9)的表达量。结论：沉默MYB表达可通过NF-κB信号抑制舌鳞癌细胞细胞活性、侵袭、迁移能力。
关键词：
舌鳞癌；侵袭；成髓细胞瘤癌基因；核因子-κB
Silencing MYB gene regulating the inhibitory effect of NF-κB signaling pathway on tongue squamous cell growth
CorrespondingAuthor: XU Wei Email: xuwei0338@163.com
DOI: 10.3978/j.issn.2095-6959.2018.11.008
Abstract
Objective: To investigate the role and molecular mechanism of myeloblastoma oncogene (MYB) in the development of tongue squamous cell carcinoma. Methods: The expression of MYB in tongue squamous cell carcinoma cell lines (UM1, CAL-27, SCC-9) and normal human oral mucosal fibroblasts (OMFbs) were detected by real-time PCR (qPCR). The cell activity and invasion and migration were analyzed by MTT and Transwell after being transfected with siRNA-MYB. Western blot was used to observe the changes of nuclear factor-κB (NF-κB) signaling pathway. Results: The expression of MYB in tongue squamous cell carcinoma cell lines (UM1, CAL-27, SCC-9) were significantly higher compared with normal human oral mucosa fibroblasts (P<0.05). Silencing MYB significantly inhibited the activity, invasion and migration of SCC-9 cells (P<0.05). Western blot analysis showed that silencing MYB expression significantly inhibited NF-κB phosphorylation and decreased matrix metalloproteinase-9 (MMP-9) expression. Conclusion: Silencing MYB expression can inhibit the activity, invasion and migration of tongue squamous cell carcinoma cells by inhibiting NF-κB signaling.
Keywords：
tongue squamous cell carcinoma; invasion; myeloblastoma oncogene; nuclear factor-κB