MicroRNA-20a对胃癌细胞生长与迁移的影响及其机制
| 作者: |
1裴洪利,
2白尚星
1 沈阳市第七人民医院检验科，沈阳 110003 2 沈阳市第六人民医院检验科，沈阳 110006 |
| 通讯: |
裴洪利
Email: 1790222693@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.11.005 |
| 基金: | 沈阳市科学技术研究与发展计划项目(20147060)。 |
摘要
目的：探讨microRNA-20a(miR-20a)对胃癌细胞的生长和迁移的影响及其相关分子机制。方法：通过qRT-PCR方法检测正常胃黏膜上皮细胞(gastric epithelial cell 1，GES-1)和胃癌细胞系MKN45，SGC7901和NUGC-3中miR-20a的相对表达水平；选取SGC7901细胞为代表，MTT法和克隆形成能力实验评估miR-20a mimic和inhibitor对细胞生长能力的影响；Transwell实验观察miR-20a的mimic和inhibitor对细胞迁移能力的影响；qRT-PCR检测miR-20a靶基因早期生长反应蛋白2(early growth reactive protein 2，EGR2)在mRNA水平的表达。结果：MiR-20a在胃癌细胞中的表达量较GES-1细胞显著增加；MTT实验、克隆形成实验和Transwell实验显示miR-20a mimic促进SGC7901细胞的生长和迁移，而miR-20a inhibitor抑制生长和迁移。MiR-20a mimic抑制EGR2 mRNA表达，而miR-20a inhibitor促进EGR2 mRNA表达。结论：MiR-20a可促进胃癌细胞生长和迁移能力，其分子机制可能与下调EGR2 mRNA的表达相关。
关键词：
microRNA-20a；胃癌；增殖；迁移；早期生长反应蛋白2
Effect of microRNA-20a on the growth and migration ability of gastric cancer cells and the underlying mechanisms
CorrespondingAuthor: PEI Hongli Email: 1790222693@qq.com
DOI: 10.3978/j.issn.2095-6959.2018.11.005
Foundation: This work was supported by the Shenyang Science and Technology Research and Development Plan Project, China (20147060).
Abstract
Objective: To explore the function and the underlying mechanism of microRNA-20a (miR-20a) on growth and migration abilities of gastric cancer cells. Methods: qRT-PCR was used to evaluate the expression levels of miR-20a in three cancer cell lines MKN45, SGC7901, NUGC-3 and human gastric epithelial cell line GES-1. Taking SGC7901 cells as an example, after transfection with miR-20a mimic and inhibitor, the ability of cellular growth were analyzed with MTT assay and clone formation assay; and the ability of and cell migration was evaluated by Transwell assay. The mRNA level of EGR2 was measured by qRT-PCR assay. Results: Compared with human gastric epithelial cell line GES-1, expression levels of miR-20a in gastric cancer cells was higher. Furthermore, miR-20a mimic promoted the growth and migration ability of SGC7901 cells, whereas miR-20a inhibitor suppressed the growth and migration ability of SGC7901 cells. Further mechanism exploration revealed EGR-2 was a direct target of miR-20a, miR-20a mimic suppressed the mRNA level of EGR-2, whereas miR-20a inhibitor promoted the mRNA level of EGR-2. Conclusion: MiR-20a enhances cell growth and migration abilities in gastric cancer cells, the mechanism may be related to down-regulating the mRNA level of EGR-2.
Keywords：
microRNA-20a; gastric cancer; proliferation; migration; early growth reactive protein 2