Objective: To access the diagnosis value of the two-dimensional (2D) and real-time three-dimensional (3D) ultrasound on different blockage sites and degrees. Methods: Two hundred and forty patients with infertility (469 fallopian tubes) examined by combined angiography and laparoscopic examination were selected. The latter was used as the gold standard to evaluate the diagnosis value of the fallopian tubes in different blockage areas and different blockage levels. Results: Ultrasound diagnosis of proximal blockage sensitivity was 91.0%, specificity 93.0%, positive prediction value 68.5%, and negative prediction value 98.4%; diagnosis of distal blockage sensitivity 69.9%, specificity 96.8%, positive prediction value 84.4%, and negative prediction value 92.8%. Ultrasound diagnostic sensitivity of fallopian tube patency was 72.9%, specificity 93.0%, positive prediction value 81.3%, and negative prediction value 89.3%; diagnostic smooth sensitivity 78.5%, specificity 84.8%, positive prediction value 75.0%, and negative prediction value 87.2%. Conclusion: 2D, real-time 3D ultrasound has a high coincidence rate in judging the patency of the fallopian tubes and the uterine laparoscopy. Combining the two methods can increase the diagnostic confidence of ultrasound doctors and provide more detailed and specific diagnostic information for clinical and patient.