长链非编码 RNA BC032469 通过调控 hTERT 影响肺癌细胞的增殖、迁移及侵袭
| 作者: |
1秦桂香,
2潘林清
1 南通市第一人民医院急诊科，江苏 南通 226001 2 连云港市妇幼保健院生殖中心，江苏 连云港 222000 |
| 通讯: |
潘林清
Email: js2018lyg@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.09.002 |
摘要
目的：探讨长链非编码RNA(long non-coding RNA，lncRNA)BC032469在肺癌细胞系中的表达及对细胞增殖、迁移和侵袭的影响。方法：采用实时荧光定量PCR(quantitative real-time PCR， qRT-PCR)测定肺癌细胞系(H1437，A549，H1299，H1975及H460)和人支气管上皮细胞(human bronchial epithelial cells，HBE)中lncRNA BC032469的表达；设计沉默lncRNA BC032469的siRNA，利用qRT-PCR检测lncRNA BC032469 siRNA在肺癌细胞中的沉默效率；CCK-8法检测细胞增殖能力的变化；划痕和Transwell试验评估细胞的迁移和侵袭能力的变化；qRT-PCR及Western印迹法分别测定hTERT mRNA和蛋白水平表达的变化。结果：LncRNA BC032469在肺癌细胞系(H1437， A549，H1299，H1975及H460)中相对表达量高于HBE(P<0.05)。沉默lncRNA BC032469后，可以抑制H460细胞的增殖、迁移和侵袭，差异有统计学意义(P<0.05)。结论：LncRNA BC032469在肺癌细胞系中高表达，沉默lncRNA BC032469抑制肺癌细胞的增殖、迁移和侵袭能力，其机制可能与下调hTERT表达有关，提示lncRNA BC032469可以作为临床肺癌治疗的潜在靶标。
关键词：
肺癌；非编码RNA BC032469；增殖；迁移；侵袭
Downregulation of long non-coding RNA BC032469 suppresses lung cancer cell proliferation, migration and invasion by regulating hTERT
CorrespondingAuthor: PAN Linqing Email: js2018lyg@sina.com
DOI: 10.3978/j.issn.2095-6959.2018.09.002
Abstract
Objective: To explore the expression of long non-coding RNA (lncRNA BC032469) in lung cancer cell lines and its effect on proliferation, migration and invasion. Methods: We compared the expression of lncRNA BC032469 in lung cancer cell lines (H1437, A549, H1299, H1975 and H460) and human bronchial epithelial cells (HBE) by quantitative real-time PCR (qRT-PCR). qRT-PCR was used to detect the silence efficiency of lncRNA BC032469 silence sequence. The proliferation, migration and invasion ability of cells were assessed by CCK-8 assay, Scratch assay and Transwell assay after the lncRNA BC032469 siRNA transfection. qRT-PCR and Western blot were used to detect the expression of hTERT. Results: qRT-PCR results showed that lncRNA BC032469 expression was higher in lung cancer cells (P<0.05). Knockdown of lncRNA BC032469 inhibited proliferation, migration and invasion of H460 cells (P<0.05). Conclusion: LncRNA BC032469 is highly expressed in lung cancer cells. Knockdown of lncRNA BC032469 inhibits lung cancer cells proliferation, migration and invasion and the mechanism may be related to the down-regulated of hTERT, which indicating that lncRNA BC032469 may be a candidate target of lung cancer gene therapy.
Keywords：
lung cancer; long non-coding RNA BC032469; proliferation; migration; invasion