文章摘要

长链非编码RNA H19，EZH2，Ki-67 及突变型P53 联合检测 在子宫颈癌诊断中的意义

作者: 1辜卫红, 2余敏敏, 1袁智民, 1吴玲, 1邱峰龙, 1项媛媛, 1方芳, 1杨慧
1 淮安市妇幼保健院妇产科，江苏 淮安 223002
2 南京市第二医院妇产科，南京 210003
通讯: 杨慧 Email: ppoouu@126.com
DOI: 10.3978/j.issn.2095-6959.2018.09.001
基金: 国家自然科学基金 (81472431)；淮安市科技计划项目 (HAS2015024)。

摘要

目的：研究长链非编码RNA(long non-coding RNA，lncRNA) H19，EZH2，Ki-67，突变型P53联合检测在子宫颈癌诊断中的意义。方法：取子宫颈活检或手术组织标本进行病检，其中50例子宫颈癌为研究组，50例非瘤子宫颈为对照组。采用qRT-PCR检测lncRNA H19在各组组织中的表达量。利用免疫组织化学SP法检测以上2组宫颈组织石蜡标本中EZH2，Ki-67，突变型P53的表达。应用SPSS 13.0软件包统计分析各组H19，EZH2，Ki-67及突变型P53的表达与子宫颈癌临床和病理诊断结果的相关性。结果：研究组H19的表达量低于对照组(P<0.05)；研究组EZH2，Ki-67，突变型P53阳性表达量高于对照组，差异具有统计学意义(P<0.05)。结论：临床工作中联合检测H19， EZH2，Ki-67，突变型P53更有利于子宫颈癌的诊断。
关键词： 子宫颈癌；长链非编码RNA H19；EZH2；核增殖抗原Ki-67；突变型P53

Expression and significance of long non-coding RNA H19, EZH2, Ki-67, and mutant P53 in cervical cancer

Authors: 1GU Weihong, 2YU Minmin, 1YUAN Zhimin, 1WU Ling, 1QIU Fenglong, 1XIANG Yuanyuan, 1FANG Fang, 1YANG Hui
1 Department of Obstetrics and Gynecology, Huaian Maternal and Child Health Care Hospital, Huaian Jiangsu 223002, China
2 Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Nanjing 210003, China

CorrespondingAuthor: YANG Hui Email: ppoouu@126.com

DOI: 10.3978/j.issn.2095-6959.2018.09.001

Foundation: This work was supported by the National Natural Science Foundation (81472431) and Science and Technology Planning Project of Huaian City (HAS2015024), China.

Abstract

Objective: To assess the expression and clinical significance of long non-coding RNA (lncRNA) H19, EZH2, Ki-67, and mutant P53 in cervical cancer. Methods: Cervical biopsy or surgical tissue specimens were divided into 2 groups according to pathological diagnosis: 50 cases of cervical cancer were regarded as an experiment group and 50 cases of non-cancerous cervical were a control group. qRT-PCR was used to evaluate the expression levels of lncRNA H19. Immunohistochemistry (SP method) was used to evaluate the expression levels of EZH2, Ki-67 and mutant P53 in fixed specimens. Data were analyzed by the SPSS 13.0 software. The associations of lncRNA H19, EZH2, Ki-67 and mutant P53 levels with clinicopathological features of cervical cancer were assessed. Results: LncRNA H19 were significantly low expressed in cervical cancer tissues compared with those of the control groups (P<0.05). EZH2, mutant P53 and Ki-67 amounts expressed positively in cervical cancer tissues. Conclusion: It is beneficial to combine the detection of lncRNA H19, EZH2, Ki-67 and mutant P53 for the diagnosis of cervical cancer.
Keywords： cervical cancer; lncRNA H19; EZH2; Ki-67; mutant P53

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