RhoGDI3在重度子痫前期胎盘中的表达及意义
| 作者: |
1刘思诗,
1陈静,
1庄艳艳,
1刘彩霞
1 中国医科大学附属盛京医院妇产科，沈阳 110004 |
| 通讯: |
刘彩霞
Email: Liucx1716@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.08.021 |
| 基金: | 国家自然科学基金(81501260)。 |
摘要
目的：探讨RhoGDI3 mRNA及蛋白在子痫前期胎盘中表达情况及意义。方法：采用qRT-PCR法检测20例重度子痫前期及17例正常妊娠胎盘组织中RhoGDI3 mRNA的表达情况，采用Western印迹法检测RhoGDI3蛋白的表达情况，采用免疫组织化学染色方法检测RhoGDI3在胎盘中的表达情况。结果：正常孕妇组胎盘中RhoGDI3 mRNA水平明显高于重度子痫前期组，为重度子痫前期胎盘的2倍(P<0.05)。RhoGDI3蛋白在正常妊娠胎盘和重度子痫前期胎盘中均有表达，且正常妊娠胎盘中的表达量为重度子痫前期胎盘的2倍(P<0.05)。免疫组织化学检测发现：正常胎盘和重度子痫前期胎盘中均有RhoGDI3的阳性表达，且主要表达在胎盘滋养细胞中。重度子痫前期组孕妇胎盘组织中RhoGDI3蛋白的阳性表达率(35.3%，6/17)，明显低于对照组(80%，16/20)。结论：RhoGDI3无论在基因水平还是蛋白水平，在重度子痫前期胎盘中的表达均减少，提示可能与重度子痫前期的发病有关。
关键词：
RhoGDI3；子痫前期；滋养细胞
Expression and significance of RhoGDI3 in maternal placenta of preeclampsia
CorrespondingAuthor: LIU Caixia Email: Liucx1716@163.com
DOI: 10.3978/j.issn.2095-6959.2018.08.021
Foundation: This work was supported by the National Natural Science Foundation of China (81501260).
Abstract
Objective: To investigate the expression of RhoGDI3 in placental tissue with preeclampsia and its relationship with the pathogenesis of preeclampsia. Methods: The expression of RhoGDI3 protein level was detected with Western bolt, RhoGDI3 mRNA was detected with real-time PCR, and the location of RhoGDI3 in the placenta was detected by immunohistochemistry, in specimens of 20 preeclampsia placentas, 17 normal human placentas. Results: We found that the expression of RhoGDI3 mRNA was stronger in normal placentas than in preeclampsia placentas, and it was twice that of the preeclampsia placentas (P<0.05). RhoGDI3 protein was expressed in the normal placentas and preeclampsia placentas, and the expression in the normal placentas was twice that of preeclampsia placentas (P<0.05). Immunohistochemistry test showed that both the normal placentas and the preeclampsia placentas had the positive RhoGDI3 expression, which was mainly expressed in the placental trophoblast. RhoGDI3 protein positive expression rate was significantly lower in preeclampsia placentas (35.3%, 6/17) than the normal placentas (80%, 16/20). Conclusion: Regardless of the level of mRNA or protein, the expression level of RhoGDI3 was stronger in normal placentas than in preeclampsia placentas, which show that RhoGDI3 may be associated with the onset of preeclampsia.
Keywords：
RhoGDI3; preeclampsia; trophoblast