Objective: To investigate the anatomical features of uterine arteriography and provide theoretical basic for the clinical uterine artery embolization. Methods: The uterine artery angiography of 115 patients with uterine artery embolization and perfusion chemotherapy from January 2013 to July 2016 were retrospectively analyzed, the anatomical features of uterine artery origins, running and branches were observed. Results: The digital subtraction angiography (DSA) data of 115 patients were collected, including 25 cases of postpartum hemorrhage, 73 cases of ectopic pregnancy and 17 other diagnostic types. The uterine arteries originated from the anterior, the posterior, the branch trunk of iliac artery. The starting section can be a sharp, right and obtuse angle. The main branches including ovarian branch, round ligament of the uterus, fallopian tube branch, cervical and vagina branch. The average diameter of uterine arteries on the left side is (2.77±0.96) mm, the right side is (2.74±0.92) mm. The average distance between the ipsilateral iliac artery bifurcation and the left uterine artery is (73.06±25.14) mm, another side is (77.67±34.62) mm. In the cases of postpartum hemorrhage, the uterine artery diameter is (2.37±0.64) mm; as for the cases of ectopic pregnancy, the diameter is (2.89±1.00) mm. The differences about the diameter of the bilateral uterine arteries in the two cases were statistically significant (P<0.05); there is no significant difference between the distance from bilateral uterine arteries to the ipsilateral iliac artery bifurcation(P>0.05); both sides of uterine artery had no significant difference in diameter (P>0.05). Conclusion: It is of important clinical significance to be familiar with the anatomical characteristics of uterine arteriography, to improve the success rate, to select the method of embolization and to reduce the complications.