蒲公英萜醇对肺癌细胞的增殖及糖酵解的影响
| 作者: |
1刘冬,
1赵鹏,
1王磊,
1兰飞,
1魏晓燕,
1张贺龙
1 空军军医大学唐都医院肿瘤科，西安 710038 |
| 通讯: |
张贺龙
Email: zh_916@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.03.001 |
摘要
目的：研究蒲公英萜醇对肺癌细胞糖酵解效应的影响。方法：采用肺癌细胞株H1299，A549检测细胞葡萄糖消耗及乳酸含量及细胞ATP，NAD+/NADH含量；同时给予血小板衍生生长因子BB(platelet-derived growth factor BB，PDGF-BB)上调Akt途径促进糖酵解反应，考察蒲公英萜醇对肺癌细胞糖酵解的影响。结果：蒲公英萜醇可明显降低肺癌细胞葡萄糖消耗、乳酸含量及细胞ATP含量，升高NAD+/NADH含量。给予PDGF刺激H1299，A549细胞后细胞葡萄糖消耗及乳酸含量显著升高，细胞ATP含量升高，己糖激酶(hexokinase，HK)活性明显升高，NAD+/NADH含量降低；给予蒲公英萜醇后，可明显降低PDGF-BB诱导的H1299，A549细胞糖酵解水平，抑制HK活性，并抑制Akt磷酸化水平；结论：蒲公英萜醇可抑制肺癌细胞糖酵解水平，抑制HK活性，该作用与调控Akt途径相关。
关键词：
肺癌；糖酵解；蒲公英萜醇
Effect of taraxerol on proliferation and glycolysis of lung cancer cells
CorrespondingAuthor: ZHANG Helong Email: zh_916@163.com
DOI: 10.3978/j.issn.2095-6959.2018.03.001
Abstract
Objective: To study the effect of taraxerol on glycolysis of lung cancer cells. Methods: We detected the concentration of cell glucose consumption, lactic acid and cellular ATP content and NAD+/NADH by lung cancer cell lines H1299 and A549. At the same time, platelet-derived growth factor BB (PDGF-BB) was given to increase the Akt pathway to promote glycolysis reaction, and to investigate the effect of dandelion terpene on the glycolysis of lung cancer cells. Results: Taraxerol significantly reduced glucose consumption, lactate and ATP concentration, and increased NAD+/NADH concentration. While simulating H1299 and A549 cells with PDGF, glucose consumption, lactic acid concentration, cellular ATP concentration and hexokinase activity increased significantly, and NAD+/NADH concentration reduced. After taraxerol was added, glycolysis induced by PDGF-BB was reduced in H1299 and A549 cells, hexokinase activity and Akt phosphorylation were inhibited. Conclusion: Taraxerol can inhibit the level of glycolysis of lung cancer cells and inhibit hexokinase activity, which is related to the regulation of Akt pathway.
Keywords：
lung cancer; glycolysis; taraxerol