Kank1基因在食管鳞状细胞癌的表达
| 作者: |
1王玻玮,
1王雯,
2马方婧,
1马遇庆
1 新疆医科大学第一附属医院病理科，乌鲁木齐 830011 2 新疆医科大学第一附属医院乳腺科，乌鲁木齐 830011 |
| 通讯: |
马遇庆
Email: 394202231@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.01.004 |
| 基金: | 新疆医科大学第一附属医院青年科学基金(2014ZRQN20)。 |
摘要
目的：研究Kank1基因在食管鳞状细胞癌中的表达差异及与临床病理学参数的关系。方法：采用免疫组织化学学法(IHC)和运用实时荧光定量PCR(qRT-PCR)技术分别检测63例食管鳞状细胞癌及其相对应的癌旁组织中Kank1 蛋白和mRNA的表达；统计分析Kank1基因与食管鳞状细胞癌患者临床病理学资料的相关性。结果：Kank1蛋白在食管鳞状细胞癌组织中低表达，阳性表达率为20%，而在相对应的癌旁组织中高表达，表达率为95%。Kank1蛋白在食管鳞状细胞癌组织中的阳性表达率显著低于其在正常组织中的阳性表达(P<0.05)；Kank1在正常食管黏膜与鳞状细胞癌组织中mRNA表达有明显差异。此外，Kank1蛋白表达与肿瘤的分化程度有相关性，与患者性别、族别、年龄、肿瘤大小、淋巴结转移无相关性。结论：Kank1在食管鳞癌组织中表达降低，提示Kank1可能参与了食管鳞状细胞的发生发展，可能是一个潜在抑癌基因。
关键词：
食管鳞癌；Kank1；抑癌基因
Kank1 gene expression in esophageal squamous cell carcinoma
CorrespondingAuthor: MA Yuqing Email: 394202231@qq.com
DOI: 10.3978/j.issn.2095-6959.2018.01.004
Foundation: This work was supported by Youth Science Project Foundation of First Affiliated Hospital of Xinjiang Medical University, China (2014ZRQN20).
Abstract
Objective: To investigate the Kank1 expression levels in esophageal squamous cell carcinoma (ESCC) and its relationship with clinicopathological data. Methods: Immunohistochemistry and real-time quantitative PCR (qRT-PCR) were used to detect the expression of Kank1 protein and mRNA in 63 cases of esophageal squamous cell carcinoma (ESCC) and corresponding adjacent tissues, respectively. The correlation between Kank1 and the clinicopathologic parameters of ESCC was statistically analyzed. Results: The positive expression rate of Kank1 protein was 95% in normal tissues, and its positive expression rate in ESCC tissues was 20%. The expression of Kank1 in ESCC tissues was significantly lower than that in normal tissues (P<0.05). The expression of Kank1 mRNA was significantly different in normal esophageal tissues and squamous cell carcinoma. In addition, the Kank1expression was significantly associated with tumor differentiation. However, Kank1 protein and mRNA had no association with gender, ethnicity, age, tumor size, lymph node metastasis. Conclusion: The Kank1 expression levels decrease in ESCC and normal tissues, suggesting that Kank1 is a potential tumor suppressor of ESCC.
Keywords：
esophageal squamous cell carcinoma; Kank1; tumor suppressor gene