Objective: To analyze the expression of miR-20b in ovarian carcinoma cell line and its effect on migration and invasion of ovarian carcinoma cell line. Methods: The expressions level of miR-20b in HO8910, A2780, SKOV3 and Hose was measured by qRT-PCR. The A2780 cell line was divided into two groups, miR-20b mimic group transfect with miR-20b mimics and negative control group transfect with miR-20b scramble by Lipofectamine 2000. The cell wound scratch assay was used to detect the ovarian cell migration ability. The cell invasion ability was detected by Transwell assay. The expression level of vascular endothelial cell growth factor (VEGF) mRNA and protein was measured by qRT-PCR and Western blot, respectively. Results: The expression of miR-20b in three ovarian carcinoma cell lines HO8910, A2780 and SKOV3 was significantly lower than normal cell line, Hose (0.30±0.05, 0.23±0.03, 0.10±0.02 vs 1.00±0.03), the difference was statistically significant (P<0.001). The wound healing rate of miR-20b mimics group was 23.20%±3.50%, while 65.70%±8.30% in negative control group, the wound healing rate was significantly lower than negative control group (P<0.001). The invasive cell number in miR-20b mimics group was 21.30±4.50, while 73.50±6.70 in negative control group, the invasive cell number in miR-20b mimics group was significantly less than that in negative control group (P<0.01). The expression level of VEGF mRNA in miR-20b mimics group was 0.30±0.02, which was significantly less than 1.00±0.05 in negative control group (P<0.001). The expression level of VEGF protein in miR-20b mimics group was 118.00±8.00, which was significantly less than 180.00±5.90 in negative control group (P<0.05). Conclusion: MiR-20b has a lower expression in ovarian carcinoma cell lines. MiR-20b inhibits migration and invasion of ovarian carcinoma cells, which may through inhibiting the expression of VEGF.