Objective: To explore the expression of enhancer of zeste homolog 2 (EZH2), MMP-2 and MMP-9 in breast cancer and to analyze their relationship with the clinical pathologic characteristics. Methods: The expression of EZH2, MMP-2 and MMP-9 protein were detected by SP immunohistochemical method in 147 breast cancer tissues, 58 normal breast tissues and 58 benign breast tumor tissues, and the relationship among the three groups were analysed. The correlation of the EZH2, MMP-2 and MMP-9 protein expression with the clinicopathological features were also analyzed. Results: Positive intensity of EZH2, MMP-2 and MMP-9 in breast cancer were significantly higher than that in normal breast tissues and benign breast tumor (P<0.001). In breast cancer the expression of EZH2, MMP-2 and MMP-9 were not correlated with patient age, menopausal status, the tumor size and histological types, but were correlated with the lymph node metastasis (P<0.001). Conclusion: EZH2, MMP-2 and MMP-9 may be closely related to the pathogenesis and progression of breast cancer. The joint detection of the 3 indictors of EZH2, MMP-2 and MMP-9 in human breast cancer tissue may contribute to cancer diagnosis and treatment, and provide a reference to the study of breast cancer.