Objective: To study the clinical efficacy and safety of traditional Chinese medicine and microwave hyperthermia combined with chemotherapy in the treatment of ovarian cancer and ascites. Methods: Eighty cases of ovarian cancer ascites were randomly divided into two groups: a control group and a treatment group, 40 cases in each group. The control group was treated with chemotherapy alone, while the treatment group was combined with traditional Chinese medicine and microwave hyperthermia on the basis of the control group. Results: All patients completed the treatment successfully, after treatment, the total effective was 27 cases (67.5%) in the control group, the treatment group was 36 (90%), the difference was statistically significant (P<0.05). After the treatment, the patient’s abdominal circumference and weight decreased than before treatment, the difference was statistically significant (P<0.05); the abdominal circumference in the treatment group decreased significantly compared with the control group, the difference was statistically significant (P<0.05); the levels of tumor markers in the two groups after treatment decreased in different degrees. The treatment group in reducing CA125, HE4 on the curative effect was prominent, the differences were statistically significant (P<0.05), which showed that the treatment group was more effective than chemotherapy alone in reducing the tumor marker. Conclusion: Traditional Chinese medicine and microwave hyperthermia combined with chemotherapy in the treatment of ovarian cancer has obvious curative effect, which can effectively control the progress of the disease, and the low side effect, and provide some clinical evidence for the treatment of ovarian cancer with ascites.