黄连素对实验性子宫肌瘤大鼠的作用及相关机制
| 作者: |
1黄亚哲,
1安爱平,
1陈建玲,
2王丽蕊,
3王秋宇,
3李晓翔,
3祁彦萍
1 南阳市中心医院生殖医学科，河南 南阳 473000 2 河北省围场县医院妇产科，河北 围场 0684501 3 南阳市中心医院妇产科，河南 南阳 473000 |
| 通讯: |
陈建玲
Email: chengluya@yeah.net |
| DOI: | 10.3978/j.issn.2095-6959.2017.08.003 |
摘要
目的:观察黄连素对大鼠子宫肌瘤的作用并探讨其初步机制。方法:建立子宫肌瘤大鼠模型。 ELISA检测各组大鼠血清中IFN-α和TNF-α含量变化;观察黄连素对血液黏度与子宫肌瘤子宫系数 的影响;HE染色法观察各组大鼠子宫形态学;免疫组织化学法观察子宫平滑肌细胞中雌激素受 体-α(estrogen receptor alpha，ER-α)含量与bcl-2蛋白表达。结果:给予黄连素处理后，子宫肌瘤大 鼠血清中IFN-α的含量显著增加，而TNF-α的含量明显降低;此外，黄连素可降低全血黏度、血浆 黏度与红细胞聚集指数，减少子宫系数与平滑肌厚度和炎性细胞浸润，抑制ER-α含量与bcl-2蛋白 表达。结论:黄连素对子宫肌瘤具有改善作用，作用机制可能于调节机体炎性因子含量水平以及 凋亡因子bcl-2有关。
关键词：
子宫肌瘤
黄连素
炎性因子
Bcl-2
Effects and mechanisms of berberine on hysteromyoma in rats
CorrespondingAuthor: CHEN Jianling Email: chengluya@yeah.net
DOI: 10.3978/j.issn.2095-6959.2017.08.003
Abstract
Objective: To investigate the effects and mechanisms of berberine on hysteromyoma in rats. Methods: Hysteromyoma model was established in rats. Then the levels of IFN-α and TNF-α were analyzed by ELISA assay. Blood viscosity and hysteromyoma coe cient were examined. e morphological changes in uterus were observed using HE staining. The expressions of ER-α and Bcl-2 in uterine smooth muscle cells were detected by immunohistochemistry. Results: After Berberine administration, IFN-α levels significantly increased, while TNF-α levels decreased. Moreover, berberine treatment decreased whole blood viscosity, plasma viscosity and erythrocyte aggregation index, while reduced hysteromyoma coefficient and inflammatory cells infiltration, and inhibited ER-α and Bcl-2 expressions in uterine smooth muscle cells. Conclusion: Berberine can improve hysteromyoma in rats, which may be likely through regulation of the levels of in ammatory factors and bcl-2.
Keywords：
hysteromyoma
berberine
in ammatory factors
Bcl-2