Bcl-2，Cox-2，HSPs和p53在骨肉瘤患者中的表达及临床意义
| 作者: |
1,2张浩强,
2李明辉,
2鲁亚杰,
1叶烁,
1田琦,
1周胜虎,
1李旭升,
2王臻
1 兰州军区兰州总医院骨科中心关节外科，兰州 730050 2 第四军医大学西京医院骨科医院骨肿瘤科，西安 710049 |
| 通讯: |
王臻
Email: wangzhen@fmmu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2017.06.016 |
| 基金: | 国家自然科学基金, 31370944，31170914 |
摘要
目的：观察分析bcl-2，Cox-2，HSPs和p53蛋白等分子生物标志物在骨肉瘤患者中的表达差异性以及相关性，为临床病理诊断和治疗提供理论依据以及指导。方法：通过对所纳入的骨肉瘤患者典型组织进行切片以及SP染色，同期20例骨软骨瘤患者作为对照组。观察bcl-2，Cox-2，HSPs和p53等蛋白分子标志物的表达，使用χ2检验分析上述蛋白表达在各组之间的差异性以及临床意义。结果：骨肉瘤患者bcl-2，Cox-2，Ki-67，HSP70，HSP90以及p53蛋白阳性表达率明显高于骨软骨瘤组，差异有统计学意义(P<0.05)；Vim蛋白的表达率在两组间差异无统计学意义(P>0.05)。结论：Bcl-2，Cox-2，HSP70，HSP90，Ki-67以及p53在骨肉瘤病灶形成以及进展过程中可能起重要的作用，并为临床病理学诊断以及精准医疗提供理论依据。
关键词：
骨肉瘤
免疫组织化学
生物标志物
p53
热休克蛋白
Expression and clinical significance of bcl-2, Cox-2, HSPs and p53 in patients with osteosarcoma
CorrespondingAuthor: WANG Zhen Email: wangzhen@fmmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2017.06.016
Abstract
Objective: To observe and analyze the difference and clinical significance of the expression of biomarkers such as bcl-2, Cox-2, HSPs and p53 in osteosarcoma tumor tissues, and explore the potential roles that the biomarker might play in the genesis and development of osteosarcoma, in order to provide the theoretical foundation for the pathological diagnosis and treatment of OS. Methods: The achieved wax tissues that with typical morphological of osteosarcoma, and then Streptavidin-Peroxidase (S-P) dyeing for the slice, the patients diagnosed as osteochondroma in the same period were selected as the control group. The expression of the protein molecular biomarker, such as bcl-2, Cox-2, HSPs and p53, were observed, the differences of the proteins expression and its clinical significance between the two groups were analyzed by Chi-square test. Results: The positive expression of bcl-2, Cox-2, HSP70/90, and p53 protein in the osteosarcoma group were significantly higher than that in the osteochondroma group (P<0.05). The expression of Vim protein had no significant difference between the two groups. Conclusion: Bcl-2, Cox-2, HSPs and Vim play important roles in the formation and progression of osteosarcoma tumor lesions, and provide the theoretical basis for pathological diagnosis and precision medicine.