GABRP基因在基底样三阴乳腺癌中的差异表达
| 作者: |
1,2王晶晶,
2张旭辉,
2修冰水,
2杨锡琴,
2应晓敏,
2刘志强,
2段翠密,
2范雅雯,
1邰艳红,
1张博,
2房涛,
2冯晓燕,
2张贺秋
1 军事医学科学院附属医院病理科，北京 100071 2 军事医学科学院基础医学研究所，北京 100850 |
| 通讯: |
张旭辉
Email: zhanghhsnow@hotmail.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.05.013 |
| 基金: | 国家重点研发计划, 2016YFC0106603 国家自然科学基金, 81672607 |
摘要
目的：探索GABRP基因在基底样三阴乳腺癌(basal-like Triple negative breast cancer，BLTNBC)中的表达，为临床提供预后诊断分子标志物。方法：通过生物信息学分析比较GABRP基因的表达；采用定量PCR方法检测GABRP基因在乳腺癌细胞系及104例乳腺癌石蜡样本中的差异表达，并采用相关性分析、卡方检验及Fisher精确概率法分析GABRP基因与细胞增殖、淋巴结转移、ER及HER-2基因表达的相关性。结果：生物信息学分析和定量PCR结果显示GABRP基因在三阴性乳腺癌(triple negative breast cancer，TNBC)及BLTNBC中显著性高表达(P<0.05)；在non-TNBC，TNBC，BLTNBC中，其表达与细胞增殖、HER-2表达无明显相关性(P>0.05)，而在BLTNBC中与淋巴结转移状态有明显相关性(P<0.05)，在non-TNBC中与ER表达有明显相关性(P<0.05)。结论：GABRP基因可作为BLTNBC的有潜力的预后诊断分子标志物。
关键词：
GABRP
三阴性乳腺癌
基底样三阴乳腺癌
Differentially expressed of GABRP genes in basal-like triple negative breast cancer
CorrespondingAuthor: ZHANG Xuhui Email: zhanghhsnow@hotmail.com
DOI: 10.3978/j.issn.2095-6959.2017.05.013
Abstract
Objective: To explore the GABRP gene level in basal-like triple negative breast cancer (BLTNBC) and elucidate molecular markers for clinical diagnosis and targeting therapy. Methods: GABRP gene expression in Metabric was analyzed through bioinformatics analysis; GABRP gene expression in breast cancer cell lines and 104 cases of breast cancer paraffin samples was detected by Q-PCR. Chi-sauqre and Fisher exact test were used to analyze the correlation between GABRP gene level with cell proliferation, lymph node metastasis and the ER and HER-2 expression. Results: The bioinformatics analysis and Q-PCR results showed that GABRP gene was highly expressed in TNBC and BLTNBC. No obvious correlation was found between GABRP gene expression with cell proliferation or HER-2 expression in non-TNBC, TNBC and BLTNBC (P>0.05), but GABRP gene expression was significantly correlated with lymph node metastasis status in BLTNBC and significantly correlated with ER expression in non-TNBC (P<0.05). Conclusion: GABRP gene could be used as a diagnosis and prognosis marker for BLTNBC.