PRSS3-mRNA与长链非编码RNA-PRSS3P2在甲状腺乳头状癌组织中的表达及其临床意义
| 作者: |
1,2何冰,
2王继纲,
2李玉军
1 青岛大学基础医学院病理学教研室，山东 青岛 266003 2 青岛大学附属医院病理科，山东 青岛 266003 |
| 通讯: |
王继纲
Email: qdwangjigang@hotmail.com 李玉军 Email: liyujun.66@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.04.003 |
| 基金: | 国家自然科学基金, 81502272 |
摘要
目的：检测PRSS3-mRNA及lncRNA-PRSS3P2在甲状腺乳头状癌(papillary thyroid carcinoma，PTC)及癌旁甲状腺组织中的表达特点，探讨二者在PTC中表达的临床病理意义。方法：收集2016年7至10月本院55例PTC及癌旁甲状腺组织的新鲜手术标本，应用荧光实时定量PCR检测PRSS3-mRNA及lncRNA-PRSS3P2在PTC和癌旁组织中表达情况，并分析其与临床病理特征的相关性。结果：PRSS3-mRNA及lncRNA-PRSS3P2在PTC组织中表达均明显高于非肿瘤组织；PRSS3P2的表达与患者的年龄相关(P<0.05)，而PRSS3与各种临床病理特征无明显相关性。结论：在PTC组织中，PRSS3-mRNA和lncRNA-PRSS3P2的表达均明显升高，这提示二者可能参与了PTC的发病过程；PRSS3-mRNA和lncRNA-PRSS3P2可以作为PTC的诊断标志物进行进一步研究。
关键词：
甲状腺乳头状癌
丝氨酸蛋白酶3
长链非编码RNA
Expression and clinical significance of PRSS3-mRNA and LncRNA-PRSS3P2 in papillary thyroid carcinoma
CorrespondingAuthor: WANG Jigang Email: qdwangjigang@hotmail.com
DOI: 10.3978/j.issn.2095-6959.2017.04.003
Abstract
Objective: To detect the expressions of PRSS3-mRNA and lncRNA-PRSS3P2 in PTCs and their corresponding normal thyroid tissues, and to explore the clinicopathologic significance of PRSS3 and PRSS3P2 in PTC. Methods: A total of 55 fresh tissues of PTC plus with their paired normal thyroid tissues resected between July 1 and October 31, 2016 were collected. The expressions of PRSS3-mRNA and lncRNA-PRSS3P2 were detected by real-time quantitative PCR. The relationship between expressions of PRSS3/PRSS3P2 and the clinicopathologic features were carefully analyzed. Results: The expression levels of PRSS3 and PRSS3P2 in PTC tissues were obviously higher than their non-tumor counterparts (P<0.05). PRSS3P2 was correlated with the age of patients (P<0.05); however, PRSS3 is not associated with the clinicopathologic features of PTC. Conclusion: The expressions of PRSS3-mRNA and lncRNA-PRSS3P2 were increased in PTC. The present findings indicate that PRSS3 and PRSS3P2 may participate the development of PTC. PRSS3-mRNA and lncRNA-PRSS3P2 can be served as two potential diagnostic markers for PTC.