结直肠癌组织中K-ras基因突变的分子病理学检测分析
| 作者: |
1许春伟,
1张博,
2魏建国,
3张立英,
1宋业颖,
1王怀涛,
1吴永芳,
1邵云,
1李晓兵,
1田玉旺
1 军事医学科学院附属医院病理科，北京 100071 2 浙江省绍兴市人民医院病理科， 浙江 绍兴 312000 3 中国人民解放军北京军区总医院病理科，北京 100700 |
| 通讯: |
许春伟
Email: xuchunweibbb@163.com 张博 Email: zenwo@qq.com 魏建国 Email: mickmouse88@163.com 张立英 Email: lyzhang1@126.com 宋业颖 Email: flxwc_cn1@163.com 王怀涛 Email: 1975klwht@sina.com 吴永芳 Email: wwwyff7950385@163.com 邵云 Email: shysep@163.com 李晓兵 Email: lxb307yy@sina.com 田玉旺 Email: blktyw@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.02.012 |
摘要
目的 应用实时荧光定量PCR方法探讨K-ras基因突变情况及其临床病理参数。方法 收集71例结直肠癌石蜡组织，使用实时荧光定量PCR法检测K-ras基因状态，突变病例采用一代测序明确突变类型。结果 结直肠癌中K-ras基因突变率为35.22%(25/71)，发现7种突变(Gly12Asp, Gly12Val, Gly12Cys, Gly12Ser, Gly12Ala, Gly12Arg和Gly13Asp)，其中1例为(Gly12Val)和(Gly12Arg)双突变，其中56.00%(14/25)的突变发生在第12密码子的第2位碱基，且最常见类型为(Gly12Asp)。K-ras基因突变率在男性组中低于女性组(χ2=7.904, P=0.005)，在无淋巴结转移组中低于有淋巴结转移组(χ2=5.851, P=0.016)，差异有统计学意义，但K-ras基因突变与其他临床病理参数（年龄、肿瘤位置、浸润深度、组织学类型及Dukes'分期）差异均无统计学意义(P>0.05)。结论 女性或有淋巴结转移结直肠癌患者K-ras基因突变多见，可作为筛查是否进行分子靶向治疗的重点人群。
关键词：
实时荧光定量PCR; 结直肠癌; K-ras基因; 突变率
The molecular pathology examination analysis of K-ras gene mutation in colorectal cancer tissue
CorrespondingAuthor: XU Chunwei Email: xuchunweibbb@163.com
DOI: 10.3978/j.issn.2095-6959.2015.02.012
Abstract
Objective To investigate K-ras gene mutation and clinical pathological parameters by application of Real-time quantitative PCR method. Methods 71 cases of colorectal cancer paraffin-embedded tissues were used Real-time quantitative PCR assay to detect K-ras status, and mutation types were made clear by the first generation sequencing. Results The K-ras gene mutation rate was 35.22%(25/71) in colorectal cancer. Seven mutation types were found, namely, Gly12Asp, Gly12Val, Gly12Cys, Gly12Ser, Gly12Ala, Gly12Arg and Gly13Asp, and one case of Gly12Val and Gly12Arg coexisting mutations were found. 56% (14/25) mutations occur at the second base of codon 12, and the most common type was Gly12Asp. The mutation rate of the K-ras gene in the male group was lower than that in the female group, and the group without lymph node metastasis was lower than the group with lymph node metastasis, the difference was statistically significant (P<0.05), but the K-ras gene mutation with other clinicopathologic parameters such as age, tumor location, depth of invasion, histological types and Dukes'stages were showed no significant differences (P>0.05). Conclusion There are more patients in the K-ras gene mutation in female colorectal cancer patients or lymph node metastasis than others, which also is expected to become an important index to judge the prognosis of colorectal carcinoma.