高表达Bcl-2下调喉癌细胞黏附能力的分子机制探讨
| 作者: |
1左建宏,
2雷明胜,
3李金成,
1文美玲,
1李赛,
1吕秀,
1王雷,
4谢金龙
1 南华大学医学院肿瘤研究所，湖南 衡阳 421001 2 张家界市人民医院呼吸内科，湖南 张家界 427000 3 邵阳医学高等专科学校内科学教研室，湖南 邵阳 422000 4 解放军第一六九中心医院肿瘤科，湖南 衡阳 421002 |
| 通讯: |
谢金龙
Email: xiejinlong1967@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.05.002 |
| 基金: | 国家自然科学基金, 81272960 湖南省科技厅重点项目资助, 2013WK2010，2014SK2015 |
摘要
目的：探讨高表达的Bcl-2对喉癌细胞黏附能力的影响。方法：建立并鉴定高表达Bcl-2的稳定细胞株，采用黏附实验检测细胞的黏附能力，流式细胞仪和Western blotting实验分析细胞表面integrin表达的变化。结果：成功构建高表达Bcl-2细胞株，下调了细胞的黏附能力，并降低了细胞表面integrin α2β1的表达，而未见对integrin αv、integrin β4、integrin α3和integrin α5的表达的影响。结论：高表达Bcl-2可能通过下调了细胞表面integrin α2β1的表达从而减低细胞的黏附能力。
关键词：
Bcl-2
黏附能力
integrin α2β1
侵袭转移
The investigation of molecular mechanism of the high expression of Bcl-2 down-regulating cell adhesion in laryngeal carcinoma cells
CorrespondingAuthor: XIE Jinlong Email: xiejinlong1967@sina.com
DOI: 10.3978/j.issn.2095-6959.2016.05.002
Abstract
Objective: To investigate the effect of high expression of Bcl-2 on the cell adhesion ability. Methods: The stable cells of high expression of Bcl-2 were built and identified. The adhesion ability was assessed by adhesion assay. The expression of integrin was analyzed by FLOW and Western blotting. Results: Bcl-2 stable cells were successfully built. Bcl-2 overexpression decreased the cell adhesion and the expression of integrin α2β1, however, it did not affect the expression of integrin αv, integrin β4, integrin α3 and integrin α5. Conclusion: High expression of Bcl-2 may reduce the cell adhesion by down-regulating the expression of integrin α2β1.