1 定州市人民医院内分泌科，河北 保定 073000
目的：研究血清miR-374b作为2型糖尿病患者(type 2 diabetes mellitus，T2DM)潜在诊断指标的可能性，并探究miR-374b在T2DM发病过程中潜在的分子机制。方法：运用qRT-PCR技术，检测30例T2DM患者及30例健康对照血清中miR-374b表达水平，测定相关生化指标，并且通过逻辑回归分析两组人群血清miR-374b对T2DM的辅助诊断价值。并且将miR-374b mimics转染进胰岛β细胞株MIN-6中，通过qRT-PCR检测miR-374b对促凋亡基因Bax和抑凋亡基因Bcl-2的mRNA水平的影响。结果：qRT-PCR分析结果显示：与健康对照组相比，miR-374b在T2DM患者的血清中水平显著升高(P<0.01)。进一步逻辑回归分析结果显示在血清中miR-374b的表达水平是T2DM独立影响因素。细胞转染实验结果显示：miR-374b能显著促进促凋亡基因Bax的mRNA表达并且抑制抑凋亡基因Bcl-2的mRNA表达。结论：血清miR-374b高表达是T2DM患者潜在的非侵入性辅助诊断标志物及独立危险因素。MiR-374b表达量升高会导致胰岛β细胞凋亡。关键词： 2型糖尿病患者；miR-374b；胰岛β细胞；荧光定量PCR
Expression of serum miR-374b expression in patients with type 2 diabetes mellitus and its potential mechanism
CorrespondingAuthor: YANG Weiran Email: firstname.lastname@example.org
Foundation: This work was supported by Medical Research Program of Hebei Province in 2019, China (20191746).
Objective: To explore the possibility of miR-374b as a potential diagnostic indicator of type 2 diabetes mellitus (T2DM) and the potential molecular mechanism of miR-374b in the pathogenesis of T2DM. Methods: Serum miR-374b expression level of 30 T2DM patients and 30 healthy controls was detected by qRT-PCR, biochemical analyzer was used to determine relevant biochemical indicators, and the auxiliary diagnostic value of serum miR-374b in the two groups was analyzed by logistic regression. Moreover, by transfecting miR-374b mimics into the pancreatic β-cell MIN-6, the effect of miR-374b mRNA levels on pro-apoptotic gene Bax and anti-apoptotic gene bcl-2 was detected by qRT-PCR. Results: qRT-PCR analysis showed that the serum miR-374b expression level in the T2DM patients was significantly higher than that of the healthy control group (P<0.01). Further logistic regression analysis showed that the serum miR-374b expression level was an independent risk factor for T2DM.Cell experiments showed that miR-374b significantly promoted the expression of pro-apoptotic gene Bax mRNA and inhibited the mRNA expression of anti-apoptotic gene bcl-2. Conclusion: The high expression of miR-374b in serum is a potential non-invasive auxiliary diagnostic marker and an independent risk factor for T2DM patients. Increased expression of miR-374b leads to apoptosis of islet β-cell.Keywords： type 2 diabetes mellitus; miR-374b; pancreatic β-cell; qRT-PCR