文章摘要

PRR11和SKA2在食管鳞癌中的表达及与临床预后的关系

作者: 1陈 洁, 1亢 春彦, 1杨 红梅, 1牛 朝霞, 1彭 蕤蕤, 2周 慧聪
1 河南医学高等专科学校病理生理学教研室,郑州 451191
2 郑州大学第二附属医院消化内科,郑州 450000
通讯: 亢 春彦 Email: liuruixuankk@163.com
DOI: 10.3978/j.issn.2095-6959.2020.02.007
基金: 河南省科技攻关资助项目(192102310103);河南省高等学校青年骨干教师培养计划资助项目(2016GGJS-261)。

摘要

目的:观察脯氨酸蛋白11(proline-rich protein 11,PRR11)和动粒相关蛋白2(spindle and kinetochore associated 2,SKA2)在食管鳞癌组织中的表达,并分析与食管鳞癌临床病理参数、预后间的关系。方法:采用免疫组织化学方法检测PRR11和SKA2在100例食管鳞癌组织及其癌旁正常组织标本中的表达,统计学分析二者与食管鳞癌临床病理参数、预后间的关系。结果:PRR11在食管鳞癌组织和癌旁正常组织中阳性表达率分别为60.00%(60/100)和17.00%(17/100),SKA2在食管鳞癌组织和癌旁正常组织中阳性表达率分别为70.00%(70/100)和37.00%(37/100),食管鳞癌组织中PRR11和SKA2阳性表达率明显高于癌旁组织,差异有统计学意义(P<0.001);PRR11和SKA2蛋白在不同TNM分期、组织分化程度及淋巴结是否转移中的表达差异均有统计学意义(P<0.05);在不同性别、年龄、肿瘤直径、不同肌层浸润、脉管浸润中表达差异无统计学意义(P>0.05);Spearman相关分析结果显示PRR11和SKA2蛋白在食管鳞癌组织中呈明显正相关(r=0.725,P<0.001);患者的无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)在PRR11和SKA2阳性表达与阴性表达之间差异均具有统计学意义(P<0.05);Cox多因素回归分析结果显示TNM分期、组织分化程度、淋巴结转移、PRR11和SKA2表达是影响食管鳞癌预后的风险因素(均P<0.05)。结论:PRR11和SKA2过表达可促进食管鳞癌的发生发展,降低食管鳞癌患者的生存期,联合监测PRR11和SKA2的表达对食管鳞癌预后的判断具有一定的临床价值。
关键词: 食管鳞癌;脯氨酸蛋白11;动粒相关蛋白2;预后

Expression of PRR11 and SKA2 in esophageal squamous cell carcinoma and its relationship with prognosis

Authors: 1CHEN Jie, 1KANG Chunyan, 1YANG Hongmei, 1NIU Zhaoxia, 1PENG Ruirui, 2ZHOU Huicong
1 Department of Pathophysiology, Henan Medical College, Zhengzhou 451191, China
2 Department of Gastroenterology, Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China

CorrespondingAuthor:KANG Chunyan Email: liuruixuankk@163.com

Foundation: This work was supported by the Project supported by the Science and Technology Research Funding of Henan Province (192102310103) and Youth Backbone Teacher Training Program of Colleges and Universities in Henan Province (2016GGJS-261), China.

Abstract

Objective: To observe the expression of proline-rich protein 11 (PRR11) and spindle and kinetochore associated 2 (SKA2) protein in esophageal squamous cell carcinoma, and to analyze the relationship between PRR11 and SKA2 protein and the clinical pathological parameters and prognosis of esophageal squamous cell carcinoma. Methods: Immunohistochemistry was used to detect the expressions of PRR11 and SKA2 in 100 cases of esophageal squamous cell carcinoma and its adjacent normal tissue samples, and the relationship between them and the clinicopathological parameters and prognosis of esophageal cancer was statistically analyzed. Results: PRR11 in esophageal squamous cell cancer tissue and normal tissue adjacent to carcinoma in the positive expression rate was 60.00% (60/100) and 17.00% (17/100), SKA2 in esophageal squamous cell cancer tissues and in normal tissue adjacent to carcinoma positive expression rate was 70.00% (70/100) and 37.00% (37/100), esophageal tissues PRR11 and SKA2 positive expression rate is significantly higher than tissue adjacent to carcinoma, statistically significant difference (P<0.05). The expression differences of PRR11 and SKA2 proteins in different TNM stages, tissue differentiation degree and lymph node metastasis were statistically significant (P<0.05). There was no significant difference in the expression of different gender, age, tumor diameter, muscle infiltration and vascular infiltration (P>0.05). Spearman correlation analysis showed that PRR11 and SKA2 protein presented significant positive correlation in esophageal squamous cell cancer (r=0.725, P<0.001). Cox multivariate regression analysis showed that TNM staging, tissue differentiation, lymph node metastasis, PRR11 and SKA2 expression were risk factors affecting the prognosis of esophageal squamous cell carcinoma (all P<0.05). Conclusion: Overexpression of PRR11 and SKA2 can promote the occurrence and development of esophageal squamous cell carcinoma and reduce the survival time of patients with esophageal squamous cell carcinoma.
Keywords: esophageal squamous cell carcinoma; proline-rich protein 11; spindle and kinetochore associated 2; prognosis