文章摘要

CTGF缺失表达抑制动脉粥样硬化易损斑块的形成

作者: 1周 心涛, 1赵 黎丙, 1闵 新文, 1陈 娇, 1张 涛
1 湖北医药学院附属东风医院心血管内科,湖北 十堰 442000
通讯: 张 涛 Email: zhangt8376@163.com
DOI: 10.3978/j.issn.2095-6959.2019.09.003

摘要

目的:通过动物模型分析探讨基于调控结缔组织生长因子(connective tissue growth factor,CTGF)表达的动脉粥样硬化易损斑块形成机制。方法:选取8~10周龄的雌性同窝CTGF+/+ApoE−/−小鼠(观察组)与CTGF−/−ApoE−/−小鼠(对照组),均建立动脉粥样硬化模型,给予高脂高胆固醇饲料喂养,观察小鼠状态,检测血脂指标,记录易损斑块的病理状况。结果:所有小鼠顺利完成实验,未观察到局部和全身性的不良反应。观察组小鼠造模后2周、4周的总胆固醇(TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、三酰甘油(TG)值比对照组高,高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)值比对照组低,差异有统计学意义(P<0.05)。两组小鼠造模后2周、4周的动脉内都形成动脉粥样硬化斑块,观察组心底、主动脉根部动脉粥样硬化斑块较对照组更显著,脂质核心面积增大,泡沫细胞显著增多;观察组小鼠造模后2周、4周的易损斑块面积都显著高于对照组(P<0.05)。观察组小鼠的MMP-9相对表达水平比对照组高(P<0.05)。结论:CTGF缺失表达可抑制MMP-9的表达,促进血脂代谢正常,从而抑制动脉粥样硬化易损斑块的形成。
关键词: 结缔组织生长因子;动脉粥样硬化;易损斑块;血脂

Lack of CTGF expression inhibits atherosclerotic vulnerable plaque progression

Authors: 1ZHOU Xintao, 1ZHAO Libing, 1MIN Xinwen, 1CHEN Jiao, 1ZHANG Tao
1 Department of Cardiovascular Medicine, Dongfeng Hospital, Affiliated to Hubei University of Medicine, Shiyan Hubei 442000, China

CorrespondingAuthor:ZHANG Tao Email: zhangt8376@163.com

Abstract

Objective: To investigate the mechanism of atherosclerotic vulnerable plaque formation based on the regulation of connective tissue growth factor (CTGF) expression by animal model analysis. Methods: The atherosclerosis model were established in 8-10 weeks old female littermate CTGF+/+ApoE−/− mice (the observation group) and CTGF−/−ApoE−/− mice (the control group). The rats were fed with high-fat and high-cholesterol diet, the state of the mice was observed, the blood lipid index was measured, and the pathological condition of the vulnerable plaques were recorded. Results: All mice were successfully completed there were no local or systemic adverse reactions were observed. The TC, TG and LDL-C values of the observation group were higher than those of the control group at 2 and 4 weeks after model establishment, and the HDL-C value were lower than that of the control group, compared the difference were statistically significant (P<0.05). The atherosclerotic plaques were formed in the arteries of the two groups at 2 and 4 weeks after modeling, the atherosclerotic plaques in the heart and aortic roots of the observation group were more significant than the control group, and the lipid core area were increased and the foam cells were increased significantly. The area of vulnerable plaques in the observation group at 2 and 4 weeks after modeling were significantly higher than that in the control group (P<0.05). The relative expression level of MMP-9 in the observation group were higher than that in the control group, and compared the difference were statistically significant (P<0.05). Conclusion: The lack of expression of CTGF signaling pathway can inhibit the expression of MMP-9 and promote the normal metabolism of blood lipids, thus inhibit the occurrence of atherosclerotic vulnerable plaque.
Keywords: connective tissue growth factor; atherosclerosis; vulnerable plaque; blood lipid