文章摘要

青蒿琥酯通过下调Rad51增加宫颈癌细胞HeLa对顺铂的敏感性

作者: 1胡 胜男, 1张 蓓, 1王 晴, 1刘 智慧, 2胡 观丽
1 南京中医药大学附属徐州市中心医院妇科,江苏 徐州 221000
2 徐州医科大学临床学院,江苏 徐州 221000
通讯: 张 蓓 Email: bettyzhang10@163.com
DOI: 10.3978/j.issn.2095-6959.2019.09.001

摘要

目的:研究青蒿琥酯和顺铂(cis-platinum,CDDP)对宫颈癌HeLa细胞的增殖及凋亡的影响及其作用机制。方法:体外培养人宫颈癌HeLa细胞,分别用青蒿琥酯(artesunate,ART)、CDDP以及两药联合处理24和48 h,采用CCK-8(Cell Counting Kit-8)、流式细胞仪检测青蒿琥酯和CDDP及两者联合应用对HeLa细胞的生长增殖和细胞凋亡作用。蛋白质印迹法检测每组细胞中重组蛋白A(recombination protein A,Rad51蛋白)表达水平;RT-PCR检测Rad51 mRNA的表达水平。结果:CCK-8及流式细胞仪结果显示与对照组相比,各给药组均可明显抑制HeLa增殖,促进细胞凋亡,差异均有统计学意义(P<0.01),联合给药组的抑制作用更加显著(P<0.001),且呈时间浓度依赖性。RT-PCR和蛋白质印迹法结果显示:与对照组相比,Rad51表达下调,联合给药组的上述相应变化更加显著,但两单独给药组之间差异无统计学意义(P>0.05)。结论:ART及CDDP均可抑制宫颈癌HeLa细胞的增殖,促进凋亡。且ART可能通过下调Rad51来增加宫颈癌HeLa细胞对CDDP的敏感性。
关键词: 青蒿琥酯;顺铂;Rad51;宫颈癌

Artesunate increases the sensitivity of cervical cancer cells to cisplatin by down-regulating Rad51

Authors: 1HU Shengnan, 1ZHANG Bei, 1WANG Qing, 1LIU Zhihui, 2HU Guanli
1 Department of Gynecology, Xuzhou Central Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Xuzhou Jiangsu 221000, China
2 Clinical College, Xuzhou Medical University, Xuzhou Jiangsu 221000, China

CorrespondingAuthor:ZHANG Bei Email: bettyzhang10@163.com

Abstract

Objective: To study the effect of artesunate and cisplatin on the proliferation and apoptosis of HeLa cells in cervical cancer and its mechanism. Methods: Human cervical cancer HeLa cells were cultured in vitro and treated with artesunate (ART), cisplatin (CDDP) and two drugs for 24 and 48 h, respectively. Cell Counting Kit (CCK-8) and flow cytometry were used to determine the growth, proliferation and inhibition of artesunate and cisplatin and their combined application on HeLa cells. Western blot was used to detect the expression level of Rad51 protein (recombination protein A) in each group. RT-PCR was used to detect Rad51 mRNA expression. Results: CCK-8 and flow cytometry showed that compared with the control group, the proliferation of HeLa was significantly inhibited in all groups, and its pro-apoptosis effect was statistically significant in each group (P<0.01), and the inhibiting effect was more significant in the combined group (P<0.001), which was also time-concentration dependent. RT-PCR and Western blot results showed that compared with the control group, Rad51 gene was down-regulated, and the corresponding changes were more significant in the combined administration group, but there was no significant difference between the two individual dosing groups (P>0.05). Conclusion: ART may increase the sensitivity of HeLa cells to CDDP by down-regulating Rad51.
Keywords: artesunate; cisplatin; Rad 51; cervical cancer