沉默KRT18 抑制软骨肉瘤细胞增殖、迁移、侵袭并促进凋亡
| 作者: |
1王硕,
1孙飞,
1徐海伟
1 枣庄矿业集团枣庄医院骨科，山东 枣庄 277100 |
| 通讯: |
王硕
Email: 3387570290@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.04.002 |
摘要
目的：探讨KRT18对软骨肉瘤细胞增殖、迁移、侵袭和凋亡的影响。方法：采用脂质体法将pcDNA 3.1，pcDNA 3.1-KRT18，siControl和siKRT18转染到软骨肉瘤细胞SW1353；采用qRT-PCR法检测细胞中KRT18的表达；Western印迹法检测细胞中KRT18蛋白表达；MTT法检测细胞的增殖率；Transwell检测细胞的迁移和侵袭；流式细胞术检测细胞的凋亡率。结果：与软骨细胞HC-A相比，软骨肉瘤细胞SW1353中KRT18的表达显著上调，且过表达KRT18可显著上调SW1353细胞增殖、迁移、侵袭，显著下调细胞凋亡(P<0.05)；沉默KRT18可抑制细胞增殖、迁移、侵袭并促进凋亡。结论：沉默KRT18可抑制软骨肉瘤细胞增殖、迁移、侵袭，促进凋亡，将可为软骨肉瘤的治疗提供新方向。
关键词：
KRT18；软骨肉瘤细胞；增殖；迁移；侵袭；凋亡
Silencing KRT18 inhibits cell proliferation, migration, invasion and promotes apoptosis of chondrosarcoma cells
CorrespondingAuthor: WANG Shuo Email: 3387570290@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.04.002
Abstract
Objective: To investigate the effects of KRT18 on cell proliferation, migration, invasion and apoptosis of chondrosarcoma cells. Methods: pcDNA3.1, pcDNA3.1-KRT18, siControl and siKRT18 were transfected into chondrosarcoma cell SW1353 by liposome method. The expression of KRT18 was detected by qRT-PCR. The protein of KRT18 was detected by Western blot. Cell proliferation rate was detected by MTT assay; cell migration and invasion were detected by Transwell; flow cytometry was used to detect cell apoptosis rate. Results: Compared with chondrocyte HC-A, the expression of KRT18 in chondrosarcoma cell SW1353 was significantly up-regulated, and overexpression of KRT18 significantly up-regulated the proliferation, migration and invasion of SW1353 cells, and significantly down-regulated apoptosis (P<0.05). Silencing KRT18 inhibited cells proliferate, migrate, invade and promote apoptosis. Conclusion: Silencing KRT18 could inhibit cell proliferation, migration, invasion and promote apoptosis of chondrosarcoma cells, which will provide a new direction for the treatment of chondrosarcoma.
Keywords：
KRT18; chondrosarcoma cells; proliferation; migration; invasion; apoptosis