儿童系统性红斑狼疮患者外周血淋巴细胞表面CD95的表达及临床意义
| 作者: |
1石海涛,
1张春莲
1 北京儿童医院顺义妇儿医院皮肤性病科，北京 101300 |
| 通讯: |
张春莲
Email: chunlianzhangbj@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.08.009 |
摘要
目的：探讨儿童系统性红斑狼疮(systemic lupus erythematosus，SLE)外周血淋巴细胞表达CD95的特征及与疾病活动性和其他免疫学指标间的关系。方法：使用流式细胞术检测60例SLE患儿和20例对照外周血T淋巴细胞亚群和B淋巴细胞表面CD95的表达，并分析其与SLE疾病活动性以及实验室检查之间的关系。结果：初发SLE患儿外周血中CD4+T细胞表面CD95的表达显著高于对照组，差异有统计学意义(P<0.05)；初发SLE患儿外周血中CD19+B细胞表面CD95的表达显著高于健康儿童(P<0.05)；CD19+CD95+ B细胞的比例和SLE疾病活动性呈正相关(r=0.4，P<0.05)；CD4+CD95+T细胞的比例和SLE疾病活动性呈正相关(r=0.3，P<0.05)，CD4+CD95+T细胞的比例和外周血抗双链DNA抗体(anti-dsDNA Abs)的水平呈正相关(r=0.2，P<0.05)；治疗后SLE患儿外周血中CD19+CD95+ B细胞和CD4+CD95+T细胞的比例均有显著下降，差异有统计学意义(P<0.05)。结论：儿童SLE患者外周血中淋巴细胞表达CD95的水平显著升高，且与SLE的疾病活动性及血清中抗双链DNA抗体相关，可以作为SLE的评价指标。
关键词：
儿童
系统性红斑狼疮
T淋巴细胞
B淋巴细胞
CD95
Expression and significance of CD95 in peripheral blood lymphocytes of children with new-onset systemic lupus erythematosus
CorrespondingAuthor: ZHANG Chunlian Email: chunlianzhangbj@126.com
DOI: 10.3978/j.issn.2095-6959.2017.08.009
Abstract
Objective: To investigate the expression of CD95 in peripheral blood lymphocytes of children with systemic lupus erythematosus (SLE) and its relationship with disease activity and other immunological parameters. Methods: Flow cytometry was used to detect the expression of CD95 on T and B lymphocytes in peripheral blood of 60 children with SLE and 20 healthy children, and analyze the relationship between the expression of CD95 and disease activity. Results: The expression of CD95 in CD4+ T cells in peripheral blood of children with SLE was significantly higher than that of healthy children. The expression of CD95 in CD19+ B in peripheral blood of children with SLE was significantly higher than that of healthy children. The proportion of CD4+ CD95+ T cells was positively correlated with SLE disease activity. The proportion of CD19+ CD95+ B cells was positively correlated with SLE disease activity. The proportion of CD4+ CD95+ T cells was positively correlated with the level of anti-double stranded DNA antibody (anti-dsDNA Abs) in peripheral blood. Conclusion: The expression level of CD95 in peripheral blood lymphocytes of children with SLE is significantly increased, and the activity of SLE is correlated with the activity of SLE and anti-double stranded DNA antibody in serum.
Keywords：
children
Systemic lupus erythematosus
T lymphocytes
B lymphocytes
CD95