Objective: To explore the method of special staining of amyloid better. Methods: Using methanol Congo red, methyl violet, Bennhola’s Freudenthal’s Congo red, Congo red, alkaline Congo red those five staining, staining to detect amyloid protein in tissue sections under microscope. Results: The larynx tissue, skin tissue, renal biopsy by methanol Congo red, methyl violet, Bennhola’s Freudenthal’s Congo red, Congo red, alkaline Congo red respectively amyloid are dyed red but its bright degree is different, color the background of different degree. Conclusion: Laryngeal tissue, skin tissue, kidney tissue in the amyloid selection of special staining method of methanol is part of Congo red positive staining the most accurate and most distinctive background color, the cleanest method.