Objective: To explore the clinicopathological features, diagnosis and differential diagnosis of prostate ductal adenocarcinoma (DAP). Methods: A total of 18 cases of DAP were reported by microscopy, immunohistochemistry, differentia diagnosis. Results: Most cases were accompanied by conventional prostate adenocarcinoma (61.35%), most of the prostate ductal adenocarcinoma cases were central type (76.92%). Histologically, tumors consist mostly of papillary, cribriform and tubular structures. Tumor cells showed tall columnar shape and nuclear atypia, abundant cytoplasm, have double color and nucleolus. Immunohistochemically results showed the tumor cell was positive for PSA and AR, P504s positive 12 cases, P63 and 34βE12positive in 1 case. The proliferation index of Ki-67 was 5%~80%. Conclusion: There are both clinicopathological and immunohistochemcial differences between the ductal carcinoma and conventional prostate adenocarcinoma. Prostate ductal adenocarcinoma should be distinguished from high grade prostate intraepithelial neoplasm, urothelial carcinoma involving the prostate and papillary adenoma of urethra.