Objective: To investigate the detection of CEACAM6 in gastric cancer and its interaction with T cells. Methods: A total of 90 patients with primary gastric cancer who underwent surgical resection from June 2012 to April 2015 in Hai’an Hospital Affiliated to Nantong University were enrolled. None of the patients received radiotherapy and chemotherapy or immunotherapy before surgery. Immunohistochemistry was used to detect the expression of CD3, CD4, CD8, CD45R0 and CEACAM6 in gastric cancer tissues of 90 patients. Results: The positive infiltration density of CD3, CD8 and CD45R0 T cells was closely related to TNM staging, differentiation and metastasis. In TNM stage I and stage II, patients with high differentiation and lymphatic metastasis were highly expressed (P<0.05). It was not related to gender, age, pathological morphology, and lesion location (P>0.05). The single-factor and multi-factor results of CEACAM6 expression were closely related to TNM stage and lymphatic metastasis, high expression in patients with stage III, IV, and lymphatic metastasis of TNM (P<0.05). It was not related to gender, age, pathological morphology, lesion location, and degree of differentiation (P>0.05). The strong positive rate of CEACAM6 was 61.11% (55/90). The high expression of CEACAM6 was negatively correlated with CD8+ T cells and CD45R0 (P<0.05), but not with CD3+ T cells and CD4+ T cells (P>0.05). There was no significant difference in survival time between different intensity of CD4 expression (P>0.05). Patients with strong positive expression of CD3, CD8 and CD45R0 had longer survival time and median survival time of 24, 26 and 29 months (P<0.05). The weak positive and negative expression of CEACAM6 had longer survival time and the median survival time was 29 months (P<0.05). Conclusion: CEACAM6 is associated with the degree of malignancy and lymphatic metastasis of gastric cancer. The expression of CEACAM6 has an inhibitory effect on tumor infiltrating T cells in gastric cancer.