姜黄素对肠缺血再灌注继发性肺损伤大鼠TNF-α 与IL-6表达的影响
| 作者: |
1廖美娟,
1赵伟成,
1梁幸甜,
1贺俭,
1刘洪珍,
1王靖,
1肖蔚
1 佛山市第一人民医院麻醉科，广东 佛山 528000 |
| 通讯: |
肖蔚
Email: zwcheng@fsyyy.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.01.001 |
| 基金: | 佛山市卫生和计划生育局医学科研课题(20160083)。 |
摘要
目的：观察姜黄素预处理对肠缺血再灌注继发性肺损伤大鼠TNF-α与IL-6表达的影响。方法：将30只清洁级大鼠随机分为3组(n=10)：假手术组(S组)，肠缺血再灌注肺损伤模型组(I/R组)，姜黄素预处理组(CUR组)。CUR组大鼠于模型建立前5 d，每天胃饲姜黄素200 mg/kg，S组与I/R组胃饲等容量生理盐水。采用夹闭肠系膜上动脉75 min后恢复灌注的方法制备肠缺血再灌注继发性肺损伤模型。恢复灌注4 h后取肺组织进行肺组织损伤评分、计算肺含水率、检测TNF-α和IL-6表达水平。结果：与S组比较，I/R组和CUR组肺组织损伤评分、肺含水率、TNF-α和IL-6表达水平升高(P<0.05)；与I/R组比较，CUR组肺组织损伤评分、肺含水率、TNF-α和IL-6表达水平下降(P<0.05)。结论：姜黄素预处理可减轻大鼠肠缺血再灌注继发性肺损伤，其机制可能与抑制肺组织炎症因子TNF-α和IL-6的表达相关。
关键词：
姜黄素；肺损伤；缺血再灌注；肿瘤坏死因子α；白介素6
Effects of curcumin pretreatment on TNF-α and IL-6 during lung injury induced by intestinal ischemia-reperfusion in rats
CorrespondingAuthor: XIAO Wei Email: zwcheng@fsyyy.com
DOI: 10.3978/j.issn.2095-6959.2018.01.001
Foundation: This work was supported by the Medical Scientific Project Foundation of Foshan Department of Health and Family Planning Bureau, China (20160083).
Abstract
Objective: To evaluate the effects of curcumin pretreatment on TNF-α and IL-6 during lung injury induced by intestinal ischemia-reperfusion in rats. Methods: Thirty female healthy SD rats were divided into three groups randomly (n=10): sham operation group (S group), lung injury inducing by intestinal ischemia-reperfusion group (I/R group), curcumin pretreatment group (CUR group). In CUR group, rats were treated intragastrically with curcumin at a dose of 200 mg/kg for 5 d before operation while rats were treated intragastrically with the same volume of NS in S group and I/R group. Lung injury induced by intestinal ischemia-reperfusion in rats was conducted by clamping the superior mesenteric artery for 75 min. The rats were sacrificed after 4-h reperfusion. The lung tissues were obtained for pathological analysis, lung water content evaluation and the determination of TNF-α and IL-6 levels. Results: The pathological scores, lung water content, and the levels of TNF-α and IL-6 were significantly increased in group I/R and group CUR as compared with group S (P<0.05). The pathological scores, lung water content, and the levels of TNF-α and IL-6 were significantly decreased in CUR group as compared with group I/R (P<0.05). Conclusion: Curcumin pretreatment attenuates the lung injury induced by intestinal ischemia-reperfusion on rats, which might be associated with inhibition of the levels of TNF-α and IL-6 in lung tissue.
Keywords：
curcumin; lung injury; ischemia reperfusion; TNF-α; IL-6