@article{LCBL36115,
author = {佳春 王 和 毅红 全},
title = {MiR-466对胃癌细胞增殖和迁移的影响及机制},
journal = {临床与病理杂志},
volume = {39},
number = {12},
year = {2019},
keywords = {},
abstract = {目的:研究miR-466对胃癌细胞系增殖和迁移的影响,并阐明其可能的机制。方法:实时聚合酶链反应 (real-time polymerase chain reaction,real-time PCR)测定胃癌细胞系AGS,BSG823,MGC-803,Hs746T及正常胃黏膜上皮细胞系GES-1中miR-466的表达。将胃癌细胞系Hs746T分成3组:miR-466过表达组(miR-466组)、阴性对照组(miR-NC组)及空白对照组(Mock组)。用LipofectamineTM 3000分别转染miR-466 mimic,miR-NC序列,Mock组为空白对照。细胞计数试剂盒-8(cell counting kit-8,CCK-8)法和细胞划痕实验分别测定3组细胞增殖和迁移能力,蛋白质印迹测定3组Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)蛋白表达。结果:miR-466在胃癌细胞系AGS,BSG823,MGC-803和Hs746T中的表达量低于正常胃黏膜上皮细胞系GES-1 (P0.05);转染后72及96 h,miR-466组OD450 nm低于miR-NC组和Mock组(P},
url = {https://lcbl.amegroups.com/article/view/36115}
}