目的：血液和尿液中的miRNAs可能存在一种潜在的无创的能够预测慢性肾脏疾病的生物学标志 物，在本研究中，我们将会研究肾结石患者血清和尿液中miR-155的表达水平。方法：选取2011年 8月~2012年8月间同济大学上海市第十人民医院泌尿外科收治的60例肾结石患者，以同期50例健 康体检者作为对照，应用实时荧光定量PCR技术检测并比较肾结石患者和健康体检者血清和尿液 miR-155的表达水平。同时计算出肾小球滤过率估算值(estimated glomerular filtration，eGFR)，通 过简单的回归分析，分析miR-155/eGFR 及miR-155/CRP表达水平之间的相关关系。结果: 肾结石 患者血清和尿液miR-155的表达水平均显著高于对照组。eGFR与尿液中miR-155的表达水平呈负 相关；CRP与尿液中miR-155的表达水平呈正相关。尿液中miR-155的表达水平同白细胞介素(IL)- 1β、 IL-6和肿瘤坏死因子(TNF)-α的表达水平呈负相关，但同正常T细胞表达分泌的活性调节因 子(regulated upon activation normal T-cell expression and secreted，RANTES)的表达水平呈正相关。 结论: 肾结石患者血清和尿液中miR-155的表达水平明显升高， miR-155表达水平的上调与eGFR下 降和CRP升高有关。我们的研究结果表明miR-155通过调节炎症因子表达在肾结石的病理生理学中 扮演重要的角色，有可能成为预测肾结石发病的生物学标志物。
Expression and its clinical significance of miR-155 in serum and urine from patients with nephrolithiasis
Objective: There may be a potential noninvasive biomarker in miRNAs of both serum and urine, which could predict the chronic kidney disease. In the present study, we will investigate the expression of miR-155 in serum and urine of nephrolithiasis and its clinical significance. Methods: Collected 60 cases blood and urine specimens of patients with nephrolithiasis from August 2011 to August 2012, and collected 50 cases blood and urine specimens of healthy volunteers in comparison. Real time quantity PCR was used to detect the expression of miR-155 in those specimens, and we compare the levels of miR-155 of nephrolithiasis and controls. Estimated glomerular filtration (eGFR) was calculated at the same time, then analyzed the correlations of miR-155/eGFR and miR-155/CRP levels by simple regression analysis. Results: The expression levels of miR-155 in serum and urine were significance higher in patients with nephrolithiasis than controls. The miR-155 levels inversely correlated with eGFR, and positively correlated with CRP. The urinary miR-155 levels significantly and inversely correlated with urinary interleukin(IL)-1β, IL-6 and tumor necrosis factor (TNF)-α levels, but significantly and positively correlated with regulated upon activation, normal T-cell expression, and secreted (RANTES) levels. Conclusion: The expression levels of miR-155 in serum and urine of nephrolithiasis patients were significantly elevated, and the elevated levels of miR-155 were correlated with the decrease of eGFR and increase of CRP. Our study suggests miR-155 may play an important role in the pathophysiology of nephrolithiasis, which may be used as a potential predictive biomarker of nephrolithiasis.