Relationship between BRAF, KRAS, NRAS, PIK3CA gene mutation and pathological characteristics in patients with colorectal cancer
Objective: To study the relationship between BRAF, KRAS, NRAS and PIK3CA gene mutations and pathological characteristics in 265 patients with colorectal cancer. Methods: From December 2014 to December 2016, tumor tissues of 265 patients with colorectal carcinoma in our hospital were retrospectively analyzed, BRAF gene (exon 15 codon 600), KRAS (gene mutations of 12,13,61 codon), NRAS (2 and 3 in exon 12 codon 13, codon 61 codons and 12 common mutations) and PIK3CA (exon 9,20) gene mutation status were detected by PCR-direct sequencing, and its relationship with clinical pathological characteristics of colorectal cancer was analyzed. Results: In 265 patients, the BRAF gene mutation rate was 6.8% (100/265), KRAS gene mutation rate was 32.1% (85/265), NRAS gene mutation rate was 5.7% (15/265), PIK3CA gene mutation rate was 11.3% (30/265). NRAS gene and KRAS gene mutation were associated with age (P<0.05), and gender, the primary site, histological type, differentiation degree, TNM staging, lymph node metastasis, distant metastasis, recurrence and metastasis were independent (P>0.05); BRAF, PIK3CA gene in the primary site for right colon in patients with mutation rate increased significantly (P<0.05), while age, gender, histological type, differentiation degree, TNM staging, lymph node metastasis, distant metastasis, recurrence and metastasis were independent (P>0.05). Conclusion: NRAS or PIK3CA has a low level in Chinese rectal cancer patients. KRAS and NRAS gene mutations were correlated with age, whereas BRAF and PIK3CA genes are correlated with the primary tumor site. A combined detection of these genes can define the pathogenesis and development of the disease.