论著 Original Article

系统性间变性大细胞淋巴瘤的免疫组织化学特征

Published at: 2017年第37卷第5期

李鑫静 1 , 陈宝珍 1 , 陈燕坪 1 , 许春伟 1 , 陈刚 1 , 郑雄伟 1
1 福建医科大学附属福建省肿瘤医院病理科,福州 350014
通讯作者 刚 陈 Email: naichengang@126.com
DOI: 10.3978/j.issn.2095-6959.2017.05.017
基金:
国家临床重点专科建设项目 2013
福建省自然科学基金 2016J01512
福建省卫生厅中青年骨干人才培养资助计划 2013-ZQN-ZD-7

摘要

目的:探讨系统性间变性大细胞淋巴瘤(anaplastic large cell lymphoma,ALCL)的免疫组织化学特征。方法:回顾性分析48例系统性ALCL的免疫组织化学和10例系统性ALCL原位杂交技术检测EBER(EBV-encoded small RNA)的结果。结果:48例系统性ALCL的肿瘤细胞均表达CD30,而间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)在41.7%(20/48)的病例阳性,其他指标阳性率为CD2为65.0%(26/40),CD3为36.2%(17/47),CD4为72.7%(16/22),CD5为42.9%(15/35),CD7为16.7%(5/30),上皮细胞膜抗原(epithelial membrane antigen,EMA)为65.6%(21/32),T细胞胞质内抗原(T-cell intracellular antigen-1,TIA-1)为79.2%(19/24),颗粒酶B(granzyme B-producing Breg,GrB)为70.0%(14/20)。所有病例的B细胞标志(CD20,PAX5,CD79α)均阴性。10例系统性ALCL有2例出现部分肿瘤性大细胞EBER阳性。结论:CD30和ALK是诊断ALCL关键及较为特异的免疫指标;有时出现人类疱疹病毒第四型(Epstein–Barr virus,EBV)感染并不能排除ALCL的诊断。


Immunohistochemical features of systemic anaplastic large cell lymphoma

Abstract

Objective: To study the immunohistochemical characteristics of systemic anaplastic large cell lymphoma. Methods: A retrospective analysis of immunophenotype of 48 cases of systemic anaplastic large cell lymphoma and the result of EBV-encoded small RNA (EBER) expression with in situ hybridization of 10 cases was performed. Results: The tumor cells expressed CD30 in 48 cases of ALCL. Anaplastic lymphoma kinase (ALK) was positive in about 41.7% (20/48) cases. The positive rates of other antigens were 65.0% (26/40) of CD2, 36.2% (17/47) of CD3, 72.7% (16/22) of CD4, 42.9% (15/35) of CD5, 16.7% (5/30) of CD7, 65.6% (21/32) of epithelial membrane antigen (EMA), 79.2% (19/24) of TIA-1, and 70.0% (14/20) of granzyme B-producing Breg (GrB). All of the cases were negative for B-cell markers (CD20, PAX-5 and CD79α). Two of 10 cases showed EBER positive in partial tumor cells. Conclusion: CD30 and ALK are the critical and relatively specific markers for ALCL; Epstein–Barr virus (EBV) involvement may not preclude the diagnosis of ALCL in some cases.


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引用

引用本文: 鑫静 李, 宝珍 陈, 燕坪 陈, 春伟 许, 刚 陈, 雄伟 郑. 系统性间变性大细胞淋巴瘤的免疫组织化学特征[J]. 临床与病理杂志, 2017, 37(5): 970-974.
Cite this article as: LI Xinjing, CHEN Baozhen, CHEN Yanping, XU Chunwei, CHEN Gang, ZHENG Xiongwei . Immunohistochemical features of systemic anaplastic large cell lymphoma[J]. Journal of Clinical and Pathological Research, 2017, 37(5): 970-974.