论著 Original Article

TaqMan探针法与尿素呼气试验法检测胃镜活检标本幽门螺旋杆菌的比较

Published at: 2017年第37卷第3期

沈和德 1 , 褚明亮 1 , 易韦 1 , 杨文秀 2
1 贵州医科大学附属人民医院病理科,贵阳 550002
2 贵州医科大学病理学教研室,贵阳 550004
通讯作者 韦 易 Email: yiwei6252@sina.com
DOI: 10.3978/j.issn.2095-6959.2017.03.005
基金:
国家自然科学基金 81560088

摘要

目的:通过比较幽门螺旋杆菌(Helicobacter pylori,Hp)感染的两种检测方法,探讨TaqMan探针法在诊断幽门螺旋杆菌感染中的价值。方法:抽取70例有完整病例资料的胃镜活检标本,进行Hp分型、鉴定检测,并将实验结果与临床的碳14尿素呼气试验和病理结果进行相关性分析。结果:Hp感染的两种检测方法(TaqMan探针法和呼气实验法)一致性程度尚可(Kappa=0.550),两种方法检测的阳性率都随病理炎症程度的加重而增加,而呼气实验法检测Hp的阳性率更高(P<0.05)。但TaqMan探针法可以将Hp进行不同毒力类型的鉴定,且TaqMan探针法可区分Hp的阳性强度(1+,2+,3+),其与病理的炎症程度有较好的相关性(γ=0.564,P<0.05)。结论:TaqMan探针法可以定性地鉴定Hp,与呼气实验法有较好的一致性。TaqMan探针法可进一步定量Hp的阳性强度,更好地反映临床的病理炎症程度。


Comparison of TaqMan PCR method and Carbon-14-Urea breath test in the diagnosis of Helicobacter pylorus

Abstract

Objective: To investigate and compare TaqMan PCR method and Carbon-14-Urea breath test on Helicobacter pylori (Hp) detection. Methods: TaqMan PCR method was used in 70 cases of clinical samples, and the results were analyzed. Results: There was no difference in the positive rate of Hp in TaqMan PCR method and Carbon-14-Urea breath test, and there was a good correlation between the two methods, but the positive rate was higher in Carbon-14-Urea breath test. Further study showed that the positive intensity of Hp can be divided into 3 types by TaqMan PCR method, and there was a good correlation between positive intensity and pathological degree of inflammation. Conclusion: TaqMan PCR method can be used as a useful molecular biological method for clinical diagnosis of Hp.


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引用

引用本文: 和德 沈, 明亮 褚, 韦 易, 文秀 杨. TaqMan探针法与尿素呼气试验法检测胃镜活检标本幽门螺旋杆菌的比较[J]. 临床与病理杂志, 2017, 37(3): 473-477.
Cite this article as: SHEN Hede, CHU Mingliang, YI Wei, YANG Wenxiu . Comparison of TaqMan PCR method and Carbon-14-Urea breath test in the diagnosis of Helicobacter pylorus[J]. Journal of Clinical and Pathological Research, 2017, 37(3): 473-477.