论著 Original Article

利胆汤对大鼠急性重症胆管炎所致的肝损伤的作用机制

Published at: 2017年第37卷第2期

王建 1 , 陈卫东 1 , 刘兴洲 1 , 吴永丰 1
1 东南大学附属中大医院江北院区,南京 210009
通讯作者 建 王 Email: wangjianliver@163.com
DOI: 10.3978/j.issn.2095-6959.2017.02.022
基金:

摘要

目的:研究利胆汤对急性重症胆管炎的大鼠所致肝损伤的作用机制。方法:选择健康的Wistar大鼠60只,3月龄,雄性,随机分组为对照组、模型组、胆宁片组、利胆汤低剂量组、中剂量组和高剂量组6组,每组10只。分别于术后立刻、术后6 h、术后12 h、术后24 h 4个时间点收集大鼠胆汁,以及检测大鼠胆总管压力;对照组和模型组灌胃给予0.9%的氯化钠;胆宁片组灌胃给予胆宁片 0.5 g/(kg·d);各利胆汤剂量组:6,12和24 g/(kg·d),灌胃给药3天后处死大鼠。HE染色观察大鼠肝病理形态;ELISA检测的方法大鼠血清中的细胞因子IL-6,TNF-α水平;Q-PCR法检测大鼠肝脏巨噬细胞移动抑制因子(migration inhibitory factor,MIF)和Toll样受体4(Toll-like receptor 4,TLR4)基因的表达。结果:利胆汤能明显的降低血清细胞因子IL-6(P<0.05)、TNF-α的表达(P<0.01),并且明显抑制MIF和TLR4的表达(P<0.01)。结论:利胆汤能够改善大鼠急性重症胆管炎所致的肝损伤,作用机制可能通过抑制MIF和TLR4的表达和下调IL-6,TNF-α的表达水平实现的。


Lidantang attenuates severe acute cholangitis induced hepatic injury

Abstract

Objective: To investigate the mechanism of lidantang for treatment of hepatic injury induced by severe acute cholangitis. Methods: Sixty male Wistar rats were randomly divided into Control group (0.9% NaCl), Model group (0.9% NaCl), Danningpian group (0.5 g/kg), and lidantang group [6, 12, 24 g/(kg·d)]. At 0, 6, 12, 24 h post-surgery, the rat bile was collected, and the pressure of duct were measured. After 3 days of intragastric administration and all rats were killed, pathological morphologic change were observed by HE staining, ELISA was used to assess the levels of serum cytokines IL-6, TNF-α, macrophage migration inhibitory factor (MIF) and Toll-like receptor 4 (TLR4) was assessed by Q-PCR. Results: Lidantang could decrease the level of serum TNF-α (P<0.01) and reduce the level of serum IL-6 (P<0.05), moreover, lidantang could also decrease the expression of MIF (P<0.01) and TLR4 (P<0.01). Conclusion: Lidantang can attenuate severe acute cholangitis induced hepatic injury via down-regulate the serum of IL-6, TNF-α and inhibit the expression of MIF, TLR4 in the liver.


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引用

引用本文: 建 王, 卫东 陈, 兴洲 刘, 永丰 吴. 利胆汤对大鼠急性重症胆管炎所致的肝损伤的作用机制[J]. 临床与病理杂志, 2017, 37(2): 353-358.
Cite this article as: WANG Jian, CHEN Weidong, LIU Xingzhou, Yongfeng WU . Lidantang attenuates severe acute cholangitis induced hepatic injury[J]. Journal of Clinical and Pathological Research, 2017, 37(2): 353-358.