论著 Original Article

上皮–间质转化在蒿甲醚逆转结直肠癌细胞放化疗抵抗中的作用

Published at: 2017年第37卷第2期

王艳俊 1 , 蒋永新 2 , 刘珊 2 , 付凤莲 1 , 王红 1
1 昆明医科大学第三附属医院云南省肺癌重点实验室,昆明 650118
2 昆明医科大学第三附属医院云南省中西医结合肿瘤临床研究中心,昆明 650118
通讯作者 永新 蒋 Email: 598104374@qq.com
DOI: 10.3978/j.issn.2095-6959.2017.02.017
基金:
云南省卫生厅项目 2014NS037
云南省教育厅基础研究项目 2016ZDX061
昆明医科大学硕士研究生创新基金项目 2016S35

摘要

目的:探讨上皮–间质转化(epithelial-mesenchymal transition,EMT)在蒿甲醚(artemether,ARE)对同期放化疗抵抗人结直肠癌细胞HCT116(HCT116CRR)的逆转作用中的作用。方法:实验分为四组:1)对照组;2)单纯放化疗组;3)ARE联合放化疗组;4)ARE组。采用Real-time PCR和Western blot,检测各组EMT相关指标E-cadherin,N-cadherin,vimentin,snail mRNA及其蛋白的表达情况。结果:Real-time PCR和Western blot结果显示E-cadherin mRNA及其蛋白的表达情况,单纯放化疗组ARE联合放化疗组>对照组>ARE组。结论:EMT在ARE逆转同期放化疗抵抗人结直肠癌细胞HCT116CRR细胞系的放化疗抵抗作用中有重要的作用,即上调E-cadherin mRNA及其蛋白的表达,下调N-cadherin,vimentin,snail mRNA及其蛋白的表达。


Effect of EMT on the reversion effect of artemether in colorectal cancer chemo-radiation resistance cell line

Abstract

Objective: To investigate the effect of epithelial-mesenchymal transition (EMT) on the reversion effect of chemo-radiation resistance of artemether (ARE) in human colorectal cancer HCT116CRR cell line. Methods: The experiment was divided into four groups: 1) Normal control group; 2) Chemoradiotherapy group; 3) ARE combined with chemoradiotherapy group; 4) ARE group. RT-PCR and Western blot was used to detect the expressions of mRNAs and proteins of E-Cadherin, N-Cadherin, Vimentin, Snail. Results: RT-PCR and Western blot results: E-cadherin mRNA and protein expression level, chemoradiotherapy group < ARE combined with chemoradiotherapy group < Normal control group < ARE group (P<0.05); N-cadherin, Snail, Vimentin mRNAs and proteins expression level, chemoradiotherapy group > ARE combined with chemoradiotherapy group > Normal control group > Artemether group (P<0.05). Conclusion: ARE plays a role of reversion to chemo-radiation resistance in human colorectal cancer HCT116CRR cell line by modulating the EMT-related molecular markers. That is to say, artemether can up-regulate the mRNA and protein of E-cadherin and down-regulate the mRNAs and proteins of N-cadherin, vimentin, and snail.


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引用

引用本文: 艳俊 王, 永新 蒋, 珊 刘, 凤莲 付, 红 王. 上皮–间质转化在蒿甲醚逆转结直肠癌细胞放化疗抵抗中的作用[J]. 临床与病理杂志, 2017, 37(2): 319-325.
Cite this article as: WANG Yanjun, JIANG Yongxin, LIU Shan, FU Fenglian, WANG Hong . Effect of EMT on the reversion effect of artemether in colorectal cancer chemo-radiation resistance cell line[J]. Journal of Clinical and Pathological Research, 2017, 37(2): 319-325.