论著 Original Article

非小细胞肺癌胸水细胞块ALK融合基因检测分析

Published at: 2016年第36卷第12期

朱礼阳 1 , 徐建平 2 , 于忠和 1 , 赵洁婷 2 , 叶伟 2 , 朱东波 2 , 孙晓 2 , 宋蓉蓉 2 , 许春伟 3
1 陆军总医院肿瘤科,北京 100700
2 安徽省胸科医院病理科,合肥 230032
3 福建省肿瘤医院病理科,福州 350014
通讯作者 忠和 于 Email: 773080192@qq.com
DOI: 10.3978/j.issn.2095-6959.2016.12.013
基金:
国家自然科学基金 81372489
北京市科技计划课题 2131100006813032

摘要

目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)胸水细胞块在间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)融合基因检测中的临床价值。方法:采用突变扩增系统PCR(ARMS-PCR)法检测215例NSCLC细胞块和404例NSCLC组织块中ALK基因的三类融合类型,并检测细胞块同时送检组织块的患者74例的一致性。结果:细胞块ALK基因融合阳性26例,阳性率12.09%(26/215);组织块ALK基因融合阳性25例,阳性率6.19%(25/404);74例有组织块对照的细胞块ALK融合基因结果一致性有67例,一致率达90.54%(67/74),其中细胞块ALK融合基因的阳性率14.86%(11/74),组织块阳性率18.92%(14/74)。结论:NSCLC胸水细胞块ALK融合基因的阳性率略高于组织块;有恶性胸水的NSCLC患者原发灶组织发生ALK融合基因阳性的概率较高。


Analysis of cell blocks ALK fusion gene in pleural effusion of non-small cell lung cancer

Abstract

Objective: To investigate the clinical value of the cell blacks for anaplastic lymphoma kinase (ALK) fusion gene detection in non-small cell lung cancer (NSCLC). Methods: 215 cases of cell block from pleural effusion of non-small cell lung cancer were collected. Three types of ALK fusion and 404 cases of tissue block were detected by ARMS-PCR method. The consistency of ALK fusion was examined in 74 cases of patients with tissue block and cell block. Results: ALK fusion was found in 26 of 215 cell blocks, the positive detection rate was 12.09%. ALK fusion was detected in 25 of 404 tissue blocks, the positive detection rate was 6.19%. 67 cases in the 74 (90.54%) cases had the same result as tissue block. ALK fusion was detected in 11 of 74 (14.86%) cell blocks, and 14 of 74 (18.92%) tissue blocks. Conclusion: The rate of ALK fusion in cell blocks of non-small cell lung cancer is higher than that in matched tissue blocks. The patients with malignant pleural effusion are likely to tend to ALK fusion.


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引用

引用本文: 礼阳 朱, 建平 徐, 忠和 于, 洁婷 赵, 伟 叶, 东波 朱, 晓 孙, 蓉蓉 宋, 春伟 许. 非小细胞肺癌胸水细胞块ALK融合基因检测分析[J]. 临床与病理杂志, 2016, 36(12): 1961-1964.
Cite this article as: ZHU Liyang, XU Jianping, YU Zhonghe, ZHAO Jieting, YE Wei, ZHU Dongbo, SUN Xiao, SONG Rongrong, XU Chunwei . Analysis of cell blocks ALK fusion gene in pleural effusion of non-small cell lung cancer [J]. Journal of Clinical and Pathological Research, 2016, 36(12): 1961-1964.