论著 Original Article

IL-17A抗体抑制变应性鼻炎鼠模型中IL-17A及RORγt并升高Foxp3 mRNA表达

Published at: 2016年第36卷第12期

顾兆伟 1 , 王韫秀 2 , 赵鹤 1 , 曹志伟 1
1 中国医科大学附属盛京医院耳鼻咽喉科,沈阳 110004
2 中国医科大学附属盛京医院医疗保险工作部,沈阳 110004
通讯作者 志伟 曹 Email: caozw@aliyun.com
DOI: 10.3978/j.issn.2095-6959.2016.12.018
基金:
国家自然科学基金 81200730
辽宁省自然科学基金项目 2014021047
辽宁省教育厅科学研究项目 LK201601
沈阳市科技局项目 F15- 199-1-41

摘要

目的:探讨IL-17A抗体对小鼠变应性鼻炎鼻黏膜中IL-17A、RORγt、Foxp3 mRNA水平的影响及意义。方法:以卵清蛋白致敏的Balb/c小鼠变应性鼻炎模型作为实验组,同期以生理盐水替代作为对照组,使用IL-17A抗体治疗作为治疗组。实时定量PCR方法检测三组小鼠鼻黏膜中IL-17A、RORγt、Foxp3mRNA的含量。结果:实验组中RORγt以及IL-17AmRNA水平均较对照组升高(P<0.05),治疗组中RORγt以及IL-17AmRNA的含量均低于实验组(P<0.05)。而实验组中Foxp3 mRNA水平较对照组下降(P<0.05),治疗组中Foxp3 mRNA的含量高于实验组(P<0.05)。结论:IL-17A抗体抑制变应性鼻炎鼠模型中IL-17A及RORγt mRNA水平并升高Foxp3 mRNA表达水平。


IL-17A antibody inhibited the IL-17A and RORγt and increased Foxp3 mRNA expression in allergic rhinitis mice

Abstract

Objective: To investigate the effect of IL-17A antibody on IL-17A, RORγt, Foxp3 mRNA in nasal mucosa of allergic rhinitis mice. Methods: BALB/C mice allergic rhinitis model was constructed with ovalbumin sensitization as the experimental group (AR group), saline was used for control group and IL-17A antibody was used for therapy group. IL-17A, RORγt, Foxp3 mRNA in nasal mucosa of the three groups were measured by using real-time PCR. Results: RORγt and IL-17A mRNA in AR group were significantly higher than those in control group (P<0.05). The expression of RORγt and IL-17A mRNA in therapy group was lower than those in AR group (P<0.05). The level of Foxp3 mRNA in AR group was lower than that in the control group (P<0.05), and the expression of Foxp3 mRNA in therapy group was higher than that in AR group (P<0.05). Conclusion: IL-17A antibody inhibited the IL-17A and RORγt mRNA levels and increased Foxp3 mRNA expression in allergic rhinitis mice. The results of this study will help to understand the pathogenesis of allergic rhinitis, which provides a theoretical basis and a new way in the targeted therapy for allergic rhinitis.


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引用

引用本文: 兆伟 顾, 韫秀 王, 鹤 赵, 志伟 曹. IL-17A抗体抑制变应性鼻炎鼠模型中IL-17A及RORγt并升高Foxp3 mRNA表达[J]. 临床与病理杂志, 2016, 36(12): 1986-1990.
Cite this article as: GU Zhaowei, WANG Yunxiu, ZHAO He, CAO Zhiwei . IL-17A antibody inhibited the IL-17A and RORγt and increased Foxp3 mRNA expression in allergic rhinitis mice[J]. Journal of Clinical and Pathological Research, 2016, 36(12): 1986-1990.