目的：探讨延胡索乙素(dl-tetrahydropalmatine，THP)对大鼠急性放射性肺损伤(irradiation induced lung injury，RILI)的保护作用。方法：成年雌性SD大鼠随机分为4组，对照组、单纯照射组(R组)、R + THP组、THP组。R组采用X射线右侧胸部单次照射15 Gy，于照射后1，4，8，12，16周分别处死大鼠，取肺组织行TUNEL及电镜检测凋亡，HE染色观察病理变化，部分肺组织行超氧化物歧化酶(superoxide dismutase，SOD)及丙二醛(malondialdehyde，MDA)检测，ELISA法测定大鼠血清TGF-β1。结果：对照组及THP组大鼠肺组织均无炎症反应。受照射组(R组、R + THP组)在照射后4~16周均表现出不同程度的肺组织细胞凋亡及RILI病理变化；但R + THP组较R组炎症反应明显减轻，R + THP组与同时段的R组比较：肺组织中MDA水平明显降低、SOD水平明显升高，血清中TGF-β1明显降低，炎性细胞募集较少。两组间不同时段血清中TGF-β1水平、肺组织中MDA，SOD水平以及肺组织细胞的凋亡指数均有显著性差异(P<0.05或P<0.01)。结论：THP能通过抑制凋亡、减轻氧化损伤、抑制TGF-β1等作用减轻大鼠急性RILI。
Tetrahydropalmatine protects against acute radiation-induced lung injury in rats
Objective: To investigate the potential radioprotective effect of tetrahydropalmatine (THP) against Irradiation induced lung injury (RILI). Methods: Sprague-Dawley rats were divided into 4 groups: a control group, an irradiation (R) group, a R + THP group and an only THP group. The rats were irradiated with a single dose of 15 Gy over their right hemithorax. After irradiation at 1, 4, 8, 12, 16 weeks, rats of each groups were killed. Lung tissues were detected by histopathologic changes with HE. Using TUNEL staining and electron microscopy to explore the effectiveness of THP displayed on irradiation induced pulmonary cells apoptosis. Another part of lung tissues were used to detect MDA and SOD level. The level of TGF-β1 in serum was detected with ELISA (P<0.05). Results: The lung of control group and THP alone group showed no inflammatory reaction. Different levels of inflammation were found in the lung tissues of the rats of R group and R + THP group in 4 to 16 weeks after irradiation, the deleterious effects of irradiation significantly ameliorated in THP group. Compared R + THP group with R group at the same period, in lung tissue, the level of MDA increased significantly, the level of SOD increased significantly, the level of TGF-β1 in serum decreased significantly, and inflammation factors was not much. The difference of the level of TGF-β1, the level of MDA and SOD in lung tissue, and the apoptosis index of lung tissue in different times between groups was statistically significant (P<0.05 or P<0.01). Conclusion: THP can markedly attenuate RILI through anti-apoptosis, anti-ﬁbrosis and anti-oxidative injury effect.