论著 Original Article

黄连素对实验性子宫肌瘤大鼠的作用及相关机制

Published at: 2017年第37卷第8期

黄亚哲 1 , 安爱平 1 , 陈建玲 1 , 王丽蕊 2 , 王秋宇 3 , 李晓翔 3 , 祁彦萍 3
1 南阳市中心医院生殖医学科,河南 南阳 473000
2 河北省围场县医院妇产科,河北 围场 0684501
3 南阳市中心医院妇产科,河南 南阳 473000
通讯作者 建玲 陈 Email: chengluya@yeah.net
DOI: 10.3978/j.issn.2095-6959.2017.08.003
基金:

摘要

目的:观察黄连素对大鼠子宫肌瘤的作用并探讨其初步机制。方法:建立子宫肌瘤大鼠模型。ELISA检测各组大鼠血清中IFN-α和TNF-α含量变化;观察黄连素对血液黏度与子宫肌瘤子宫系数的影响;HE染色法观察各组大鼠子宫形态学;免疫组织化学法观察子宫平滑肌细胞中雌激素受体-α(estrogen receptor alpha,ER-α)含量与bcl-2蛋白表达。结果:给予黄连素处理后,子宫肌瘤大鼠血清中IFN-α的含量显著增加,而TNF-α的含量明显降低;此外,黄连素可降低全血黏度、血浆黏度与红细胞聚集指数,减少子宫系数与平滑肌厚度和炎性细胞浸润,抑制ER-α含量与bcl-2蛋白表达。结论:黄连素对子宫肌瘤具有改善作用,作用机制可能于调节机体炎性因子含量水平以及凋亡因子bcl-2有关。


Effects and mechanisms of berberine on hysteromyoma in rats

Abstract

Objective: To investigate the effects and mechanisms of berberine on hysteromyoma in rats. Methods: Hysteromyoma model was established in rats. Then the levels of IFN-α and TNF-α were analyzed by ELISA assay. Blood viscosity and hysteromyoma coefficient were examined. The morphological changes in uterus were observed using HE staining. The expressions of ER-α and Bcl-2 in uterine smooth muscle cells were detected by immunohistochemistry. Results: After Berberine administration, IFN-α levels significantly increased, while TNF-α levels decreased. Moreover, berberine treatment decreased whole blood viscosity, plasma viscosity and erythrocyte aggregation index, while reduced hysteromyoma coefficient and inflammatory cells infiltration, and inhibited ER-α and Bcl-2 expressions in uterine smooth muscle cells. Conclusion: Berberine can improve hysteromyoma in rats, which may be likely through regulation of the levels of inflammatory factors and bcl-2.


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引用

引用本文: 亚哲 黄, 爱平 安, 建玲 陈, 丽蕊 王, 秋宇 王, 晓翔 李, 彦萍 祁. 黄连素对实验性子宫肌瘤大鼠的作用及相关机制[J]. 临床与病理杂志, 2017, 37(8): 1565-1570.
Cite this article as: HUANG Yazhe, AN Aiping, CHEN Jianling, WANG Lirui, WANG Qiuyu, LI Xiaoxiang, QI Yanping . Effects and mechanisms of berberine on hysteromyoma in rats[J]. Journal of Clinical and Pathological Research, 2017, 37(8): 1565-1570.