目的：观察黄连素对大鼠子宫肌瘤的作用并探讨其初步机制。方法：建立子宫肌瘤大鼠模型。ELISA检测各组大鼠血清中IFN-α和TNF-α含量变化；观察黄连素对血液黏度与子宫肌瘤子宫系数的影响；HE染色法观察各组大鼠子宫形态学；免疫组织化学法观察子宫平滑肌细胞中雌激素受体-α(estrogen receptor alpha，ER-α)含量与bcl-2蛋白表达。结果：给予黄连素处理后，子宫肌瘤大鼠血清中IFN-α的含量显著增加，而TNF-α的含量明显降低；此外，黄连素可降低全血黏度、血浆黏度与红细胞聚集指数，减少子宫系数与平滑肌厚度和炎性细胞浸润，抑制ER-α含量与bcl-2蛋白表达。结论：黄连素对子宫肌瘤具有改善作用，作用机制可能于调节机体炎性因子含量水平以及凋亡因子bcl-2有关。
Effects and mechanisms of berberine on hysteromyoma in rats
Objective: To investigate the effects and mechanisms of berberine on hysteromyoma in rats. Methods: Hysteromyoma model was established in rats. Then the levels of IFN-α and TNF-α were analyzed by ELISA assay. Blood viscosity and hysteromyoma coefficient were examined. The morphological changes in uterus were observed using HE staining. The expressions of ER-α and Bcl-2 in uterine smooth muscle cells were detected by immunohistochemistry. Results: After Berberine administration, IFN-α levels significantly increased, while TNF-α levels decreased. Moreover, berberine treatment decreased whole blood viscosity, plasma viscosity and erythrocyte aggregation index, while reduced hysteromyoma coefficient and inflammatory cells infiltration, and inhibited ER-α and Bcl-2 expressions in uterine smooth muscle cells. Conclusion: Berberine can improve hysteromyoma in rats, which may be likely through regulation of the levels of inflammatory factors and bcl-2.