论著 Original Article

人经血来源间充质干细胞对小鼠A549肺癌化疗效果的影响研究

Published at: 2016年第36卷第12期

丁浩 1 , 王剑 1 , 贾方平 1 , 易晶 1
1 江苏大学附属人民医院呼吸内科,江苏 镇江 212004
通讯作者 剑 王 Email: 706713396@qq.com
DOI: 10.3978/j.issn.2095-6959.2016.12.008
基金:
江苏省自然科学基金资助项目 BK2012705
镇江市社会发展项目 SH2013045
江苏科技大学临床医学发展基金 JLY2010006

摘要

目的:探讨人经血来源间充质干细胞(mesenchymal stem cells,MenSCs)对小鼠A549肺癌化疗效果的影响。方法:取20只BALB/C裸小鼠建立肺癌模型,随机分为实验组和对照组各10只;对照组仅给予化疗干预,实验组则于化疗期间将某机构赠予的MenSCs经DiI荧光标记后于鼠尾静脉(3×105/只)进行注射。肺癌造模后14 d,两组小鼠均断颈椎处死并收集其肿瘤,比较两组小鼠瘤体体积、瘤体质量、抑瘤率及双肺湿重、肿瘤转移率、肿瘤转移个数、抑制转移率差异。结果:20只BALB/C裸小鼠均进行统计,无脱落报告。20只小鼠接瘤后第7天在右前肢腋下均可触及瘤体长出,两组小鼠瘤体生长曲线比较,差异无统计学意义(P>0.05)。两组小鼠的平均瘤体体积和平均瘤体质量比较,差异均无统计学意义(P均>0.05);抑瘤率为8.4%。实验组小鼠双肺湿重、肿瘤转移率及肿瘤转移个数均显著低于对照组小鼠,差异有显著性(P<0.05);抑制转移率为71.8%。结论:MenSCs协同化疗在抑制小鼠肺癌肿瘤转移方面有一定效果。


Influence of human blood derived mesenchymal stem cells on chemotherapy in mice with A549 lung cancer

Abstract

Objective: To investigate the influence of human blood derived mesenchymal stem cells (MenSCs) on the effects of chemotherapy in mice with A549 lung cancer. Methods: Twenty BALB/C nude mice were taken for establishing lung cancer model, and were randomized into experimental group and control group with 10 mice in each group. The control group only received chemotherapy intervention. The experimental group was treated with tail vein injection of DiI fluorescent labeled MenSCs given by an institution (3×105/mouse). After 14 days modeling of lung cancer, mice in the two groups were killed by breaking the cervical spine and the tumors were collected. The tumor volume, tumor mass, tumor inhibition rate, double lung wet weight, tumor metastasis rate, number of tumor metastasis and metastasis inhibition rate were compared between the two groups. Results: In this experiment, 20 BALB/C nude mice were statistically analyzed, and there was no report about cases lost to follow up. On the 7th day after inoculated tumor, there were tumors in 20 mice under the right fore limb armpit. There was no significant difference in tumor growth curve between the two groups (P>0.05). There was no significant difference in the average tumor volume and average tumor weight between the two groups (P>0.05); the tumor inhibition rate was 8.4%. The double lung wet weight, tumor metastasis rate and the number of tumor metastasis in the experimental group were significantly lower than those in the control group (P<0.05); the inhibition rate was 71.8%. Conclusion: MenSCs combined with chemotherapy have certain effects in inhibiting pulmonary tumor metastasis.


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引用

引用本文: 浩 丁, 剑 王, 方平 贾, 晶 易. 人经血来源间充质干细胞对小鼠A549肺癌化疗效果的影响研究[J]. 临床与病理杂志, 2016, 36(12): 1933-1937.
Cite this article as: DING Hao, WANG Jian, JIA Fangping, YI Jing . Influence of human blood derived mesenchymal stem cells on chemotherapy in mice with A549 lung cancer[J]. Journal of Clinical and Pathological Research, 2016, 36(12): 1933-1937.