Expression and clinical significance of long non-coding RNA uc.339 in non-small cell lung cancer
Objective: To investigate the expression of uc.339 and its association with clinicopathologic features in non-small cell lung cancer (NSCLC). Methods: The total RNA in 30 fresh specimens of NSCLC and the adjacent normal lung tissues were extracted respectively. The expressions of uc.339 in the tissues were detected by real-time quantitative PCR. The difference and its relationship with clinicopathological features were analyzed. The total RNA in the centrifugation precipitation of 20 specimens of NSCLC pleural effusions and 20 specimens of non-cancer pleural effusions were extracted respectively. The expressions of uc.339 were detected by real-time quantitative PCR. Expressions of P53 in NSCLC tissues were detected by immunochemistry. Results: The expression level of uc.339 in NSCLC tumor tissues was much lower than that in the normal tissues (P<0.05), which was significantly correlated with clinical stage (P<0.05), while was not associated with the histological type, age and gender of the patients (P>0.05). A strongly negative correlation was observed between uc.339 and p53 expression (P<0.05). The expression level of uc.339 in malignant pleural effusions was significantly lower than that in the benign pleural effusions (P<0.05). Conclusion: uc.339 might act as an anti-oncogene in NSCLC, its mechanism may be related to p53 signaling pathways. uc.339 plays a role in tumor makers, prognosis and target therapy.