论著 Original Article

两种突变特异性抗体用于肺腺癌EGFR基因突变的评价

Published at: 2016年第36卷第10期

卢建平 1 , 师怡 2,3 , 何银珠 2 , 陈宝珍 2 , 王晓江 2 , 周冬梅 1 , 许春伟 1,2 , 陈刚 1,2,3
1 福建医科大学教学医院,福建省肿瘤医院病理科,福州 350014
2 福建医科大学教学医院,福建省肿瘤医院分子病理室,福州 350014
3 福建省肿瘤转化医学重点实验室,福州 350014
通讯作者 刚 陈 Email: naichengang@126.com
DOI: 10.3978/j.issn.2095-6959.2016.10.017
基金:
国家临床重点专科建设项目 2013
福建省卫生系统中青年骨干人才培养项目 2015-ZQN-JC-08

摘要

目的:验证EGFR 19delE746-A750和21L858R突变抗体的灵敏度和特异性以及免疫组织化学与RT-PCR方法的一致性,并探讨免疫组化在肺腺癌EGFR基因突变状态评价中的应用。方法:采用免疫组织化学方法对139例经过RT-PCR验证EGFR基因突变状态的病例进行检测。并采用SPSS19.0软件对两种方法的检测结果进行一致性分析。结果:与RT-PCR结果相比,EGFR delE746-A750和L858R突变抗体的总体敏感性为78.5%,特异性为93%。分别分析EGFR delE746-A750和L858R特异抗体,前者的敏感性和特异性分别是64.3%和97.8%,后者为90.3%和95.2%。采用SPSS19.0软件进行Kappa检验显示免疫组化和RT-PCR的结果高度一致。结论:EGFR delE746-A750和L858R突变抗体具有良好的灵敏度和特异性,结合全自动免疫组化仪进行EGFR基因突变检测是一种经济便捷可靠的方法。


Evaluation of EGFR mutations in lung adenocarcinoma with two mutation specific antibodies

Abstract

Objective: To verify the sensitivity and specificity of the two EGFR mutation antibodies: 19delE746-A750 and 21L858R, and to discuss the application of immunohistochemistry in evaluating the EGFR mutations in lung adenocarcinoma. Methods: 139 cases were detected by immunohistochemistry which the EGFR mutation status were verified by RT-PCR firstly, then analysis the consistency of the results by software SPSS19.0. Results: Compared to the results of RT-PCR, the overall sensitivity and specificity of EGFR delE746-A750 and L858R mutation antibodies were 78.5% and 93%. The sensitivity and specificity of EGFR delE746-A750 mutation antibodies was 64.3% (former) vs. 90.3% (the latter), and the sensitivity and specificity of EGFR L858R mutation antibodies was 97.8% (former) vs. 93% (the latter). The results of immunohistochemical and RT-PCR were highly consistent analysed by SPSS19.0. Conclusion: The EGFR delE746-A750 and L858R mutation antibodies have a good sensitivity and specificity, combined with automatic immune-histochemical method for EGFR mutation detection which is economical and convenient and reliable.


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引用

引用本文: 建平 卢, 怡 师, 银珠 何, 宝珍 陈, 晓江 王, 冬梅 周, 春伟 许, 刚 陈. 两种突变特异性抗体用于肺腺癌EGFR基因突变的评价[J]. 临床与病理杂志, 2016, 36(10): 1552-1557.
Cite this article as: LU Jianping, SHI Yi, HE Yinzhu, CHEN Baozhen, WANG Xiaojiang, ZHOU Dongmei, XU Chunwei, CHEN Gang . Evaluation of EGFR mutations in lung adenocarcinoma with two mutation specific antibodies [J]. Journal of Clinical and Pathological Research, 2016, 36(10): 1552-1557.