论著 Original Article

睾丸孤核受体4(TR4)促进巨噬细胞向前列腺癌浸润

Published at: 2016年第36卷第10期

刘鹏举 1 , 秦勇 1 , 朱进 2 , 刘渊 1 , 顾海 1 , 梅方 1 , 丁献凡 1
1 杭州市红十字会医院泌尿外科,杭州 310000
2 苏州大学附属第二医院泌尿外科,江苏 苏州 215000
通讯作者 献凡 丁 Email: xianfan023@sina.cn
DOI: 10.3978/j.issn.2095-6959.2016.10.011
基金:
杭州市卫生科技计划(一般)项目 201450029

摘要

目的:研究前列腺癌TR4水平对巨噬细胞浸润的影响。方法:用transwell体系进行巨噬细胞THP-1迁移实验,在人体组织中研究睾丸孤核受体4(Testicular orphan receptor 4,TR4)水平和巨噬细胞标志物CD36的关系。结果:过表达TR4促进了巨噬细胞向前列腺的迁移;敲低TR4抑制了巨噬细胞向前列腺癌的迁移;人体前列腺癌组织标本中TR4较高的患者,CD36表达较高。结论:前列腺癌的TR4可能促进了巨噬细胞浸润。


Testicular orphan receptor 4 (TR4) promotes human macrophage infiltration in prostate cancer

Abstract

Objective: To investigate the role of testicular orphan receptor 4 (TR4) in the recruitment of human macrophage THP-1 cells in prostate cancer. Methods: Transwell system was used in the study of cellular migration to measure the THP-1 recruitment ability of prostate cancer (PCa) cells with varying levels of TR4; human PCa tissue was collected to study the relation of TR4 levels and CD36 levels by IHC staining. Results: Over-expression of TR4 increased THP-1 migration in PCa cells, knocking-down TR4 could decrease the THP-1 migration in PCa cells; IHC staining demonstrated that TR4 levels positively correlate with CD36 levels. Conclusion: TR4 may promote THP-1 cells infiltration in prostate cancer.


comments powered by Disqus

全文

引用

引用本文: 鹏举 刘, 勇 秦, 进 朱, 渊 刘, 海 顾, 方 梅, 献凡 丁. 睾丸孤核受体4(TR4)促进巨噬细胞向前列腺癌浸润[J]. 临床与病理杂志, 2016, 36(10): 1522-1525.
Cite this article as: LIU Pengju, QIN Yong, ZHU Jin, LIU Yuan, GU Hai, MEI Fang, DING Xianfan . Testicular orphan receptor 4 (TR4) promotes human macrophage infiltration in prostate cancer[J]. Journal of Clinical and Pathological Research, 2016, 36(10): 1522-1525.