非小细胞肺癌胸水细胞块ROS1融合基因检测分析
| 作者: |
1朱礼阳,
2徐建平,
1于忠和,
2赵洁婷,
2叶伟,
2朱东波,
2孙晓,
2宋蓉蓉,
3许春伟
1 陆军总医院肿瘤科，北京 100700 2 安徽省胸科医院病理科，合肥 230032 3 福建省肿瘤医院病理科，福州 350014 |
| 通讯: |
于忠和
Email: 773080192@qq.com 许春伟 Email: xuchunweibbb@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.11.007 |
| 基金: | 国家自然科学基金, 81372489 北京市科技计划课题, 2131100006813032 |
摘要
目的：探讨非小细胞肺癌胸水细胞块在ROS1(c-ros oncogene 1，receptor tyrosine kinase)融合基因突变检测中的临床价值。方法：采用ARMS-PCR法检测215例非小细胞肺癌胸水细胞块和404例非小细胞肺癌组织块中ROS1基因融合的四种类型，并检测细胞块同时送检组织块的患者74例的一致性。结果：细胞块ROS1融合基因阳性7例，阳性率3.26%(7/215)；组织块ROS1融合基因阳性8例，阳性率1.98%(8/404)；74例有组织块对照的细胞块ROS1融合基因结果一致性有71例，一致率达95.95%(71/74)，其中细胞块ROS1融合基因的阳性率2.70%(2/74)，组织块阳性率6.76%(5/74)。结论：非小细胞肺癌胸水细胞块ROS1融合基因的阳性率略高于组织块；有恶性胸水的非小细胞肺癌患者原发灶组织发生ROS1融合基因阳性的概率较高。
关键词：
肺肿瘤
非小细胞肺癌
细胞块
ROS1
Analysis of cell blocks ROS1 fusion gene in pleural effusion of lung adenocarcinoma
CorrespondingAuthor: YU Zhonghe Email: 773080192@qq.com
DOI: 10.3978/j.issn.2095-6959.2016.11.007
Abstract
Objective: To investigate the clinical value of the cell blacks for ROS1 (c-ros oncogene 1, receptor tyrosine kinase) fusion gene detection in non-small cell lung cancer (NSCLC). Methods: Selected 215 cases of cell block from pleural effusion of NSCLC and 404 cases of tissue block of NSCLC, detected the four types of ROS1 fusion by ARMS-PCR method. The consistency of ROS1 fusion was examined in 74 cases of patients with tissue block and cell block. Results: ROS1 fusion was found in 7 of 215 cell blocks, positive detection rate of 3.26% (7/215). ROS1 fusion was detected in 8 of 404 tissue blocks (positive detection rate of 1.98%). 71 cases in the 74 (95.95%) cases had the same result as tissue block. ROS1 fusion was detected in 2 of 74 (2.70%) cell blocks, and 5 of 74 (6.76%) tissue blocks. Conclusion: The rate of ROS1 fusion in cell blocks of non-small cell lung cancer is higher than in matched tissue blocks. The patients with malignant pleural effusion are likely tend to ROS1 fusion.