Objective: To investigate the effects of different anesthesia methods on the expression of inflammatory factors and stress factors in patients with spinal tuberculosis during perioperative period. Methods: A systematic analysis of spinal tuberculosis patients in our hospital from January 2014 to 2016 January was carried out. 86 cases of patients who underwent surgical removal of the lesion were included in this study. All of the patients were divided into group A and group B according to the condition of the them, in which a group of 43 cases used epidural block combined with intravenous anesthesia and the remaining 43 cases as group B only in intravenous anesthesia way. Serum levels of perioperative IL-1, IL-6 and IL-10 were analyzed in two groups of patients. Results: The serum levels of IL-1 and IL-6 in the two groups increased gradually from T0 phase to T3 phase, and reached the peak in the period of T3, and T4 period began to decrease. The serum levels of IL-6 and IL-10 in the two groups were significantly higher than that in the T4 phase, and the difference was statistically significant (P<0.05). The levels of T1 and T0 in the serum of the groups were significantly higher than that in the group. Between the two groups comparison can be found, a group of patients in the T2 and T3 in serum IL-1 beta and IL-6 levels were significantly lower than those in group B, and group A patients in stage T2 to T4 in serum IL-10 levels were significantly higher than those of the group B, the difference is statistically significant (P<0.05). Conclusion: Compared with intravenous anesthesia, epidural anesthesia combined with intravenous anesthesia can help to reduce the inflammatory reaction and improve the prognosis after surgery, which is worth promoting in clinical practice.