Objective: To explore the localization and different expression of sorcin in mouse hepatocarcinoma ascites cell lines with different metastatic potentials and the role of sorcin in tumor lymphatic metastasis. Methods: Mouse hepatocarcinoma cell lines Hca-F with high lymphatic metatastic potential and Hca-P with low lymphatic metastatic potential were used. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to analyze the expression of sorcin in Hca-F/Hca-P cell lines. Western blot was performed to detect the level of protein expression of sorcin in Hca-F/Hca-P cell lines. Immunofluorescence assay was used to detect the localization and expression of sorcin in cells. Cell proliferation was assessed by cell counting kit-8. the Boyden-Transwell chamber assay was performed to analyze the cell migration ability and cell invasion ability. Results: The proliferation, migration and invasion ability of Hca-F cells was significantly higher than those of Hca-P cells in vitro; the mRNA and protein expression of sorcin in Hca-F cells were 1.5 and 1.8 folds higher than that in Hca-P cells, respectively; sorcin mainly expressed in cytoplasm of the above 2 cell lines. Conclusion: The expression of sorcin is high in Hca-F cell lines and it locates in cytoplasm. Sorcin may play an important role in tumor proliferation, migration and invasion, which lays the foundations for further investigation of the underlying mechanisms.