姜黄素诱导黑素瘤细胞凋亡的研究
| 作者: |
1王红梅,
1韩正祥,
1杜秀平,
1李韬涛,
2蒋冠
1 徐州医学院附属医院肿瘤科，江苏 徐州 221000 2 徐州医学院附属医院皮肤科，江苏 徐州 221000 |
| 通讯: |
王红梅
Email: wanghongmei8179@126.com 韩正祥 Email: cnhzxyq@163.com 杜秀平 Email: xiupingdu@yahoo.com.cn 李韬涛 Email: litt@126.com 蒋冠 Email: 5716589@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.09.015 |
摘要
目的：研究姜黄素诱导黑素瘤细胞A375、M14凋亡的机制。方法：将对数生长的黑素瘤细胞A375、M14分别用姜黄素干预；显微镜下观察黑素瘤细胞株A375和M14的凋亡状态；应用Western Blot方法检测凋亡相关蛋白Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB的变化；应用流式细胞仪检测细胞凋亡率。结果：实验发现姜黄素干预黑素瘤细胞后可见细胞发生凋亡；在姜黄素诱导黑素瘤细胞凋亡过程中，凋亡相关蛋白Bax和γ-H2AX表达水平较对照组升高，而Bcl-2、NF-κB表达水平较较对照组下降，有活性的Caspase-3生成；随着姜黄素的浓度增加，黑色素瘤细胞早期凋亡的比例增加。结论：姜黄素诱导黑素瘤细胞生长凋亡的机制可能与其对凋亡相关蛋白Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB的表达调控有关。
关键词：
姜黄素
黑素瘤
凋亡
Curcumin results melanoma cells apoptosis
CorrespondingAuthor: WANG Hongmei Email: wanghongmei8179@126.com
DOI: 10.3978/j.issn.2095-6959.2015.09.015
Abstract
Objective: To observe the effects of curcumin on the apoptosis of two melanoma cell lines A375 and M14. Methods: Cell apoptosis in human melanoma cell lines A375 and M14 treated with curcumin was measured by morphological method. Furthermore four apoptosis related proteins: Bcl-2, Bax, Caspase-3, γ-H2AX and NF- κB were determined by Western Blotting analysis. Apoptosis rate were detected through FCM (flow cytometry). Results: The data showed that curcumin can induce tumor cell apoptosis. Western blotting analysis confirmed that curcumin could express increasing proteins Bax and γ-H2AX, down-regulating the proteins Bcl-2, and NF-κB, as well as, activation of Caspase-3. It showed that with the increase of the concentration of curcumin, melanoma cell early apoptosis ratio increased. Conclusion: This study showed that curcumin exhibited a remarkably increased apoptosis-inducing effects in melanoma cells.