上皮–间质转化在蒿甲醚逆转结直肠癌细胞放化疗抵抗中的作用
| 作者: |
1王艳俊,
2蒋永新,
2刘珊,
1付凤莲,
1王红
1 昆明医科大学第三附属医院云南省肺癌重点实验室，昆明 650118 2 昆明医科大学第三附属医院云南省中西医结合肿瘤临床研究中心，昆明 650118 |
| 通讯: |
蒋永新
Email: 598104374@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.02.017 |
| 基金: | 云南省卫生厅项目, 2014NS037 云南省教育厅基础研究项目, 2016ZDX061 昆明医科大学硕士研究生创新基金项目, 2016S35 |
摘要
目的：探讨上皮–间质转化(epithelial-mesenchymal transition，EMT)在蒿甲醚(artemether，ARE)对同期放化疗抵抗人结直肠癌细胞HCT116(HCT116CRR)的逆转作用中的作用。方法：实验分为四组：1)对照组；2)单纯放化疗组；3)ARE联合放化疗组；4)ARE组。采用Real-time PCR和Western blot，检测各组EMT相关指标E-cadherin，N-cadherin，vimentin，snail mRNA及其蛋白的表达情况。结果：Real-time PCR和Western blot结果显示E-cadherin mRNA及其蛋白的表达情况，单纯放化疗组ARE联合放化疗组>对照组>ARE组。结论：EMT在ARE逆转同期放化疗抵抗人结直肠癌细胞HCT116CRR细胞系的放化疗抵抗作用中有重要的作用，即上调E-cadherin mRNA及其蛋白的表达，下调N-cadherin，vimentin，snail mRNA及其蛋白的表达。
关键词：
蒿甲醚
结直肠癌
肿瘤细胞
放化疗抵抗
上皮–间质转化
Effect of EMT on the reversion effect of artemether in colorectal cancer chemo-radiation resistance cell line
CorrespondingAuthor: JIANG Yongxin Email: 598104374@qq.com
DOI: 10.3978/j.issn.2095-6959.2017.02.017
Abstract
Objective: To investigate the effect of epithelial-mesenchymal transition (EMT) on the reversion effect of chemo-radiation resistance of artemether (ARE) in human colorectal cancer HCT116CRR cell line. Methods: The experiment was divided into four groups: 1) Normal control group; 2) Chemoradiotherapy group; 3) ARE combined with chemoradiotherapy group; 4) ARE group. RT-PCR and Western blot was used to detect the expressions of mRNAs and proteins of E-Cadherin, N-Cadherin, Vimentin, Snail. Results: RT-PCR and Western blot results: E-cadherin mRNA and protein expression level, chemoradiotherapy group < ARE combined with chemoradiotherapy group < Normal control group < ARE group (P<0.05); N-cadherin, Snail, Vimentin mRNAs and proteins expression level, chemoradiotherapy group > ARE combined with chemoradiotherapy group > Normal control group > Artemether group (P<0.05). Conclusion: ARE plays a role of reversion to chemo-radiation resistance in human colorectal cancer HCT116CRR cell line by modulating the EMT-related molecular markers. That is to say, artemether can up-regulate the mRNA and protein of E-cadherin and down-regulate the mRNAs and proteins of N-cadherin, vimentin, and snail.