HIF-1和CD24在结肠癌组织中的表达及其意义
| 作者: |
1丁秀杰,
2张睿
1 辽宁省肿瘤医院 核医学科，沈阳 110042 2 辽宁省肿瘤医院 结直肠科，沈阳 110042 |
| 通讯: |
张睿
Email: zzzrrr1234@yeah.net |
| DOI: | 10.3978/j.issn.2095-6959.2014.06.017 |
| 基金: | 辽宁省科技厅自然科学基金, 2013020158 |
摘要
目的：检测HIF-1和CD24蛋白在结肠癌组织中表达，探讨HIF-1和CD24蛋白与结肠癌患者预后的关 系。方法：收集25例明确诊断的结肠癌组织及其相应癌旁组织，提取总蛋白。采用Western-blot方 法检测HIF-1和CD24蛋白在结肠癌组织和癌旁组织中的含量。利用Pearson相关性分析评估HIF-1和 CD24蛋白表达的相互关联。Kaplan-Meier生存曲线分析HIF和CD24蛋白与结肠癌患者预后的相关 性。结果：HIF-1和CD24蛋白在结肠癌组织中的含量明显高于癌旁组织(P<0.05)。HIF-1与患者的 性别、年龄、肿瘤分化、淋巴侵袭、静脉侵袭、肿瘤大小和病理分期因素无关。CD24与患者的性 别、淋巴侵袭、静脉侵袭、肿瘤大小和病理分期因素无关。但与患者年龄和肿瘤分化两个因素相 关。Kaplan-Meier生存曲线分析显示同时表达HIF-1和CD24的患者比单独表达HIF-1或CD24的患者 预后更差。结论：HIF-1和CD24蛋白在结肠癌组织中表达异常升高，并且与结肠癌患者预后相关。
关键词：
结肠癌
CD24
HIF-1
预后
病理特征
The role of HIF-1 and CD24 in colorectal cancer
CorrespondingAuthor: ZHANG Rui Email: zzzrrr1234@yeah.net
DOI: 10.3978/j.issn.2095-6959.2014.06.017
Abstract
Objective: To determine the HIF-1 and CD24 expression in colorectal cancer and investigate the relationship between the expression of HIF-1 and CD24 and the prognosis of the patients with colorectal cancer. Methods: The tissues of cancer center and peritumoral of esophageal carcinoma were collected by surgery. The levels of HIF-1 and CD24 proteins were detected by using Western-blot. The correlation between CD24 protein and HIF- 1 protein was evaluated by using Pearson correlation analysis. Kaplan-Meier survival curve analysis was used to analysis the relationship of CD24 and HIF-1 protein with the prognosis of patients with colon cancer. Results: The levels of HIF-1 and CD24 expression were higher in cancer tissues than that in normal tissues (P<0.05). HIF-1 expression was not associated with sex, age, differentiation, lymphatic invasion, venous invasion, tumor size and pN category. CD24 expression was not associated with sex, lymphatic invasion, venous invasion, tumor size and pN category, but related with age of patient and tumor differentiation. The survival rate of the patients with both HIF-1 and CD24 expression was lower than the patients with HIF-1 or CD24 expression by using Kaplan-Meier survival curve analysis (P<0.05). Conclusion: Abnormal high HIF-1 and CD24 expression was observed in colorectal cancer tissues, which suggest that there was association between HIF-1 or CD24 and the development of colorectal cancer.