Objective: To summarize the experience of radical gastrectomy operation of Da Vinci system, evaluate its effect and application value. Methods: The clinical and pathological data of 250 patients with gastric cancer in our department from Nov 2011 to Nov 2014 were retrospectively analyzed. A total of 126 patients who experienced robotic gastric cancer and 124 patients who underwent Laparoscopic gastric cancer enrolled in this study. The operative data and short-term postoperative outcomes were compared between the groups. Results: Compared with the laparoscopic group, the robotic group had less intraoperative blood loss, larger numbers of retrieved lymph nodes and longer operation time, and the differences were statistically significant (P<0.05). There were no significant differences in gastrointestinal function recovery time, postoperative hospital stay, and complications between the groups (P>0.05). During a mean follow-up period of 17.2 (range, 3-30) months, 10 patients experienced relapse and metastasis, 3 patients died of the disease in robotic group, while 12 patients experienced relapse and metastasis, 2 patients died of the disease in laparoscopic group. Conclusion: With da Vinci robotic system, the standard D2 gastric cancer radical surgery can be accomplished successfully. It is feasible and safe, and the clinical effect is reliable.